Trigonella Foenum-Graecum protects mouse macrophage derived raw cell line 264.7 from advanced glycosylation end products with promising anti-aging potential | [Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23 • 2023]

Author(s):

1. Laila Anwer Ali: Department of Pharmacology, Faculty of Pharmacy, Hamdard University, Karachi, Pakistan

2. Syed Abid Ali: HEJ Research Institute of Chemistry, ICCBS, University of Karachi, Pakistan

3. Farzana Sadaf: Department of Pharmacology, Faculty of Pharmacy, Hamdard University, Karachi, Pakistan

4. Faheema Siddique: Department of Pharmacology, DOW University of Health Sciences, Karachi, Pakistan

5. Syed Waleed Ahmed Bokhari: Department of Pharmacognosy, Hamdard University, Karachi, Pakistan

6. Ghulam Abbas: Department of Pharmacology, Ziauddin University, Karachi, Pakistan

Abstract:

Aging is described as a gradual decline in the efficiency of biochemical and physiological progressions till death. Several theories have been introduced to define aging process. Among which theory related to accumulation of advanced glycation end products in the organism causing senescence. Accumulation of glycated macromolecules, chiefly proteins known to be a tissue damaging factor linked to aging or age-related disorders. Formation of advanced glycation end products (AGEs) and macromolecular damage also expedited by increase in oxidative stress due to reactive oxygen species. Our study design assess the inhibitory activity of Trigonella foenumgraecum extract on formation of advanced glycation end products (AGEs) and free radical scavenging activity (ROS). On cellular viability RAW 264.7 cell line having RAGE (receptor of advanced glycation end products) on its surface were assessed using MTT assay. Results of Anti-glycation assay revealed that AGEs remarkably reduced the cell viability. Cytoprotective effects were seen in dose dependent manner, in Fenugreek seed extract (FgSE) at doses of 0.1, 0.5 and 1 mg/ml using Aminoguanidine (AG) as positive control. Data strongly suggests that Fenugreek seeds delays the process of aging in vitro. DPPH assay of FgSE reveal maximum inhibitory activity at 1mg/kg.

Page(s): 294-294

DOI: DOI not available

Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023

Keywords:

MTT assay , antiaging , advanced glycation end products AGEs , Fenugreek seed extract FgSE , cell viability

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