Abstract:
Citrus fruit is widely known as the most significant fruit globally and it has a crucial function in sustaining human nutrition. Pakistan has historically relied on it as a significant source of foreign exchange. Unfortunately, a number of abiotic and biotic diseases affect both its production and quality, such as citrus canker (CC) induced by Xanthomonascitripv. citri (Xcc) is most devastated. Xcc bacterium is one of the most well-known plant pathogenic bacteria that are responsible for infections in citrus, cotton, and rice. Due to the existence of several Xcc races, no resistant variations against this disease are currently available. The study was performed to explore the morpho-genetic characterization of the Xcc causal organism of CC. A systematic survey of randomly chosen areas in the districts of Dera Ghazi Khan, Bahawalpur and Multan were done for collection of disease samples showing distinctive canker symptoms and carried out into lab for isolation of bacterium. Various confirmatory tests using standard protocol such as gram staining and KOH test were resulted all the isolates are gram negative bacterium. Koch's postulates were followed and found Xcc is the causal agent of citrus canker. Molecular characterization of Xcc was performed through DNA extraction and commercially sequenced after identification. Some nearly associated sequences were downloaded through BLAST on NCBI and form a phylogenetic tree. This phylogenetic tree showed all the strains examined belong to the Xcc strains. For the management of citrus canker, nine antibiotics (Amoxcillin, Kanamycin, Erythromycin, Azithromycin, Vibramycin, Ampicillin, Clindamycin, Streptomycin and Clarithromycin) were tested in in-vitro conditions to assess their efficacy against Xcc. Among the tested antibiotics ampicillin at a concentration of 700 (ppm) showed the maximum effectiveness in preventing the development of Xcc.
Page(s):
77-77
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023