Alpha Mangosteen effect on MDA Level and the Pancreatic Morphology Rattus norvegicus (Berkenhout, 1769) Induced by Alloxan | [Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences • 2020]

Author(s):

1. Devyana Dyah Wulandari: Department of Medical Laboratory Technology, Faculty of Health, Nahdlatul Ulama Surabaya University,Jl. Jemursari, 51-57, Surabaya, East Java, 60237,Indonesia

2. Zumrotul Mufidah: Department of Medical Laboratory Technology, Faculty of Health, Nahdlatul Ulama Surabaya University,Jl. Jemursari, 51-57, Surabaya, East Java, 60237,Indonesia

3. Taslim Ersam: Department of Chemistry, Faculty of Science, Sepuluh Nopember Institute of Technology,Jl. Keputih, Sukolilo, Surabaya, East Java, 60111,Indonesia

4. Devyani Diah Wulansari: Department of Pharmacy, Faculty of Pharmacy, University of Surabaya,Jl. Tenggilis Mejoyo No.12, Rungkut, Surabaya, East Java, 60293,Indonesia

5. Alfredo Vita Corpuz: Department of Medical Laboratory Technology, University of Northern Philippines,Tamag, Vigan City, Ilocos Sur, 2700,Philippines

Abstract:

The alpha mangosteen compound is a single compound isolated from rind extract Garcinia mangostana Linn, known to have antidiabetic bioactivity. This research aimed to determine the effect of the a-mangosteen compound on Malondialdehyde (MDA) and rats pancreatic tissue induced with type 2 Diabetes mellitus. Twentyfour Wistar male rats were divided into four groups, each group consisting of six rats. The first group was set as a positive control, rats injected with a single dose of 150 mg kg-1 alloxan. The second group was the negative control, which means that no treatment. The third group was injected with a single dose of 150 mg kg-1 alloxan and a-mangosteen compound 10 mg kg-1, while the last group was injected with a single dose of 150 mg kg-1 alloxan and 10 mg kg-1 standard drug glibenclamide. All groups were monitored for 3 wk, and blood sugar levels were measured using a Glucose meter. Blood samples were taken for the measurement of MDA, and the pancreas organ was removed for histological study. This research showed that the diference of MDA level between a-mangosteen compound treatment and glibenclamide was only 0.481 8. It means that the a-mangosteen compound can reduce MDA levels alike the standard drug glibenclamide capabilities. Islets of Langerhans on normal pancreatic mice (control (-)) looked iflling full with the endocrine cells spread throughout the pancreas. While at the control group (+) there was a roomempty space (fewer the number of beta cells) in islets of Langerhans. On the treatment groups, those were giving a-mangosteen, and drug compounds standard (glibenclamide) showed improvement in Langerhans ß cells compared with a treatment group of diabetic mice (control+). The administration of a-mangosteen compound 10 mg kg-1 BW able to decrease MDA level 1.204 7 nmol dl-1. While based on histological observations of the pancreas showed that the alpha mangosteen treatment group showed improvement of Langerhans ß cells compared to the diabetic rat group (positive control).

Page(s): 15-21

DOI: DOI not available

Published: Journal: Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, Volume: 57, Issue: 4B, Year: 2020

Keywords:

Diabetes Mellitus , lipid peroxidation , Garcinia mangostana L , Beta cells

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