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Unveiling the production, purification, and multifaceted potential of lysozymes: a review
Author(s):
1. Syeda Quratulain Gillani: Department of Biotechnology, University of Sialkot,Sialkot-51311,Pakistan
2. Komal Waheed: Department of Biotechnology, University of Sialkot,Sialkot-51311,Pakistan
3. Sabuh Najam: Department of Biotechnology, University of Sialkot,Sialkot-51311,Pakistan
4. Noaf Nadeem: Department of Biotechnology, University of Sialkot,Sialkot-51311,Pakistan
5. Laiba Jameel: Department of Biotechnology, University of Sialkot,Sialkot-51311,Pakistan
6. Komal Imran: Department of Biotechnology, University of Sialkot,Sialkot-51311,Pakistan
Abstract:
Lysozyme is a versatile enzyme that has garnered significant recognition due to its multitude of applications in diverse fields, such as pharmaceutical, healthcare, cosmetics, food, and agriculture industry. It is naturally derived from avian egg whites and subsequently produced using microbial systems such as bacteria, fungi, and yeast. Lysozyme is used in food preservation to inhibit the growth of microorganisms that cause food spoilage, thus extending shelf life and ensuring food safety. In therapeutics, it acts as an anticancer, antimicrobial and antiviral agent, with potential for combating life threatening diseases. Furthermore, lysozyme has also been used to improve gut health in livestock, thereby improving overall health of animals. Industrial production of lysozyme using microbial sources involving genetic engineering has modified the synthesis method, making it more stable, economical, and improved functionalities. Advanced purification techniques, involving precipitation, ion-exchange chromatography, and ultrafiltration yield meticulously refined product with high purity, and enzyme activity. Moreover, the advancement of lysozyme-based nanomaterial has enhanced antimicrobial effectiveness and facilitated targeted delivery in biomedical applications. This review article provides insights onto the lysozyme's sources, production, purification, and extensive applications, to encourage further research into its untapped potential.
Page(s): 225-233
DOI: DOI not available
Published: Journal: International Journal of Biology and Biotechnology, Volume: 22, Issue: 2, Year: 2025
Keywords:
antimicrobial , Anticancer , food preservation , antimicrobial , genetic engineering , nanotechnology , Animal feed , Lysozyme
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