An efficient, short and cost-effective regeneration system for transformation studies of sugarcane (Saccharum officinarum L.). | [Pakistan Journal of Botany • 2009]

Author(s):

1. Azad Hussain Shah: School of Biological Sciences, University of the Punjab, New Campus, Lahore, Pakistan

2. Naeem Rashid: School of Biological Sciences, University of the Punjab, New Campus, Lahore, Pakistan

3. M. Saleem Haider: School of Biological Sciences, University of the Punjab, New Campus, Lahore, Pakistan

4. Faiza Saleem: School of Biological Sciences, University of the Punjab, New Campus, Lahore, Pakistan

5. M. Tahir: School of Biological Sciences, University of the Punjab, New Campus, Lahore, Pakistan

6. Javed Iqbal: School of Biological Sciences, University of the Punjab, New Campus, Lahore, Pakistan

Abstract:

Sugarcane genetic transformation efforts are seriously hampered by the lack of an efficient and reproducible regeneration system. An efficient, short and cost-effective regeneration system, through direct embryogenesis, was developed for local cultivars and elite lines of sugarcane. Using 1-2 mm thick meristematic young leaf whirls, direct embryogenesis was achieved in Murashige & Skoog (MS) medium supplemented with 2, 4-dichlorophenoxyacetic acid (2, 4-D) under cool white fluorescent light for 16 hour/day at 25 ± 2 °C within three weeks. Of the various concentrations of 2, 4-D tested, 3 mg/L induced the highest frequency of embryogenic callus (60%). The embryos germinated in the fourth week on the same medium. Shoot proliferation and multiplication was carried out in liquid MS medium containing benzyl aminopurine (BAP) at a concentration of 1 mg/L. The improved regeneration system will particularly be useful in our ongoing genetic transformation studies.

Page(s): 609-614

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 41, Issue: 2, Year: 2009

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