Abstract:
Mango is an important tropical and subtropical tree fruit crop (Mangifera indica), with a very significant financial impact and belongs to the variety Mangifera and genus Anacardiaceae. Due to its numerous health benefits, including a lower risk of heart disease, resistance to malignant growth, and resistance to viral activities, the fruit is rich in cellular reinforcements and is advised to be included in a regular diet. SSRs, which are effective markers, have been used to identify different mango cultivars and reveal the variation in quality. The objective of this work was to perform comparative genomic and transcriptome analysis of mango fruit quality and flavonoid characteristics like aroma and flavor.Seven cultivars of mango were taken, and DNA was extracted. The quality and quantity were checked through PCR by applying primers. Gel electrophoresis was done to check the DNA bands. Genomic data, microsatellite identification, motif imperfection, repeat length analysis, and digital expression profiling were identified by bioinformatics analysis. In investigations of genetic diversity simple sequence Repeat (SSR) or microsatellite markers have shown to be incredibly helpful tools. Approximately 100 of these markers, the bulk of which are di- or tri-nucleotide, have been discovered in Mangifera indica to date. Motif imperfection analysis and gene expression profiling in the mango genome and the analysis of microsatellite conservation imprints in mango evolution were facilitated by the comparative genomics technique.The comparative genomics technique was used to explore the distinctive correlations between repeat density, motif imperfection analysis, and gene expression profiling of the fruit quality genes in the mango genome. This study summarizes the research findings like genome exploration, chromosome-wise repeat density, motif imperfection, Circos plot visualization and heat maps. By doing PCR, the cultivar Summer Bahishst Chaunsa with primer no. 2 showed bands on gel electrophoresis, so this primer will be helpful in the future for genetic selection. According to our findings, the abundance of imperfect microsatellites in the genome was closely associated with motif imperfection in repeat length.
Page(s):
74-74
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023