Thermal stability of purified superoxide dismutase from liver of commercially valuable fish, Labeo rohita Exposed to Pb+Cr mixture | [Pakistan Journal of Agricultural Sciences • 2021]

Author(s):

1. Huma Naz: Department of Zoology, Cholistan University of Veterinary and Animal Sciences,Bahawalpur,Pakistan

2. Sajid Abdullah: Department of Zoology, Wildlife and Fisheries, University of Agriculture,Faisalabad,Pakistan

3. Tanveer Ahmed: Department of Life Sciences, Khwaja Fareed University of Engineering and Information Technology,Rahim Yar Khan,Pakistan

4. Khalid Abbas: Department of Zoology, Wildlife and Fisheries, University of Agriculture,Faisalabad,Pakistan

5. Muhammad Umar Ijaz: Department of Zoology, Wildlife and Fisheries, University of Agriculture,Faisalabad,Pakistan

6. Muhammad Adeel Hassan: Department of Parasitology, Cholistan University of Veterinary and Animal Sciences,Bahawalpur,Pakistan

7. Muhammad Anjum Zia: Department of Chemistry and Biochemistry, University of Agriculture,Faisalabad,Pakistan

Abstract:

In this experiment, effect of lead (Pb) + chromium (Cr) mixture on superoxide dismutase (SOD) in the liver of Labeo rohita at a concentration of 11.1 mgL-1 was observed. The ammonium sulphate precipitation and ion exchange chromatography techniques were successfully used to purify SOD. After purification, SOD activity of control and Pb+Cr treated fish was noted as 581.00 and 645.45 UmL-1, respectively while the specific activity was 1383.33 and 1613.62 Umg-1, respectively. The fold purification value of SOD was 2.75 and 2.45 for control and stressed fish, respectively. The recovery was calculated as 77.06 and 57.43% for control and stressed fish, respectively. The results of kinetic characterization showed that SOD form control and exposed fish had maximum activity at pH 6.5 and 7.0. Temperature also had a significant effect on activity of SOD. The SOD activity was measured maximum at 30°C for both control and Pb+Cr exposed fish. The Km value of liver SOD for control and Pb+Cr treated L. rohita was calculated as 1.48 and 0.62 mM, respectively. The value of Vmax for SOD from liver of control and Pb+Cr exposed fish was 1000 and 570 U mL-1, respectively. The enthalpy of denaturation (?H*) for liver SOD from control and Pb+Cr exposed L. rohita was computed as 3.492 and 2.802 KJ mol-1 at 40°C, respectively and these values were dropped off with increasing the temperature until it remains 3.251 and 2.561 KJ mol-1 at 70°C, respectively. The free energy of thermal denaturation (?Gº) of liver SOD was slightly increased with increasing temperature until 75°C which shows its resistance against heat. The values of ?Gº was observed as 58.03 and 57.95 KJ mol-1 for control and exposed fish at 40°C, respectively while the same was increased upto 62.37 and 62.00 KJ mol-1 at 70°C, respectively. It was concluded from negative value of ?S* (entropy of inactivation) that the SOD is stable thermodynamically.

Page(s): 1191-1196

DOI: 10.21162/PAKJAS/21.9770

Published: Journal: Pakistan Journal of Agricultural Sciences, Volume: 58, Issue: 4, Year: 2021

Keywords:

Characterization , SOD , fish , purification , heavy metals mixture

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views