Tlr2 and Tlr4 as a Biomarker in Adult and Children Iraqi Bacterial Sepsis Syndrome Patients. | [Pakistan Journal of Biotechnology • 2018]

Author(s):

1. FarahQ Younis: Biology Department, College of Science, University of Mustansiriya, Iraq

2. Ali Hussein Alwan: Biology Department, College of Science, University of Mustansiriya, Iraq

3. Neihaya H Zaki: Biology Department, College of Science, University of Mustansiriya, Iraq

Abstract:

This study aimed to investigate the possibility using of TLR2 and TLR4 gene expression as an early biomarkers for diagnosis bacterial septic syndrome in children and elderly. The causative agents of infection was determined by blood culture. TNF-a, IL-10, and sHLA-DR were measured by ELISA and TLR2, TLR4 expression was determined by quantitative RT-PCR. about 75 patients was diagnosed with sepsis syndrome included in this study . The age range of patients (13days-92years) with mean 56.3±13.9 and matched to 55 healthy volunteers. Depending on age, patients were divided into 4 age groups (group A 40 year). The results indicated that the levels of TNF-a, IL-10 and sHLA-DR in all age groups were significantly increased except the level of TNF-a in group B compared with control groups. A positive correlation has observed between levels of TNF-a and sHLA-DR with patients age respectively. TLR2 and TLR4 mRNA expression was significantly increased in all age groups with significant difference between group A and groups D. TLR2 expression highly increases in G+ve infection while TLR4 expressed highly in G-ve bacteria. can be conclude that TLR2 and TLR4 expression in bacterial sepsis patients indicates the strong possibility of using them as biomarkers in the early diagnosis of bacterial sepsis syndrome, in children and elderly patients

Page(s): 837-842

DOI: DOI not available

Published: Journal: Pakistan Journal of Biotechnology, Volume: 15, Issue: 3, Year: 2018

Keywords:

Keywords are not available for this article.

References:

[1] Akira , S.,K.Takeda, 2004.Toll-like receptor signalling,Nat. Rev. Immunol 4 499 -511

[2] Angus , D.C.,Linde-Zwirble , J.,Lidicker , G. Clermont, J.,Carcillo and M.R.,Pinsky, 2001.Epidemiology of severe sepsis in the United States: analysis of incidence, outcome, and associated costs of care,Crit Care Med 29 1303 -10

[3] Artero , A.,R.Zaragoza,J.M.Nogueira, 2012.Epidemiology of severe sepsis and septic shock. In Severe Sepsis and Septic Shock-Understanding a Serious Killer,InTech -

[4] Bruunsgaard , H.,P.Skinhøj,J.Qvist,B.K.Pedersen, 1999.Elderly humans show prolonged in vivo inflammatory activity during pneumococcal infections,J. Infect. Dis 180 551 -4

[5] Burrell , A.R.,M.L.McLaws,M.Fullick,R.B.Sullivan,D.Sindhusake, 2016.early intervention saves lives,Med. J. Aust 73 -

[6] Das U.N., 2000.Critical advances in septicemia and septic shock,Critical care 4 290 -

[7] De Gaudio , A,S.Rinaldi,C.Chelazzi,T.Borracci, 2009.Pathophysiology of sepsis in the elderly: clinical impact and therapeutic considerations,Curr Drug Targets 10 60 -70

[8] Hietbrink , F., 2008.Kinetics of the excessive cellular innate immune response after injury (Doctoral dissertation, -

[9] House , R.V.,J.Descotes, 2007.Cytokines in human health: immunotoxicology, pathology and clinical applications,Humana 366 -

[10] Kleiner , G.,A.Marcuzzi,V.Zanin,L.Monasta,G.Zauli, 2013.Cytokine levels in the serum of healthy subjects,Mediators Inflamm -

[11] Kotgire , S. and S.,Hatkar, 2017.Aerobic bacteriological profile and its antimicrobial sensitivity pattern from blood culture specimens in a tertiary care hospital,APALM 4 A23 -27

[12] Krabbe , K.S.,M.Pedersen,H.Bruunsgaard, 2004.Inflammatory mediators in the elderly,Exp. Gerontol 39 687 -99

[13] Kumar , H., T.,Kawai and S.,Akira, 2011.Pathogen recognition by the innate immune system,Int Rev Immunol 30 16 -34

[14] Lu , Y.C., 2009.Regulation of toll-like receptor signal transduction pathways, Doctoral dissertation, -

[15] Perry , S.E., S.M.,Mostafa , R.,Wenstone , A.,Shenkin and P.J. McLaughlin, 2004.HLA-DR regulation and the influence of GM-CSF on transcription, surface expression,Int. J. Med. Sci 126 -

[16] Rebmann , V., S.,Ugurel , W.,Tilgen , U.,Reinhold and H.,Grosse‐W andilde, 2002.Soluble HLA‐DR is a potent predictive indicator of disease progression in serum from early‐stage melan-oma patients,IJC 100 580 -5

[17] Rittirsch , D.,M.A.Flierl,P.A.Ward, 2008.Harmful molecular mechanisms in sepsis,Nat Rev Immunol 776 -

[18] Rudd , K.E.,L.K.Tutaryebwa,T.E.West, 2017.2): e0171422 (,PloS one 12 -

[19] Sahoo , D.,L.Mohanty,S.S.Panda,S.N.Mishra, 2016.Bacteriological analysis of blood culture isolates in patients with sepsis in a tertiary care hospital of eastern India,IJCMR 3 3448 -50

[20] Simon , A.K.,G.A.Hollander,A.McMichael, 2015.Evolution of the immune system in humans from infancy to old age,In Proc. R. Soc. B 20143085 -

[21] Starr , M.E,H.Saito, 2014.Sepsis in old age: review of human and animal studies,Aging Dis 2 -

[22] Thevaranjan , N.,A.Puchta,C.Schulz,Age-Associated Microbial Dysbiosis Promotes Intestinal Permeability , Systemic Inflammation, and Macrophage,Dysfunction, 2017.,Cell Host & Microbe 21 455 -66

[23] Wacker , C.,A , Prkno,F.M.Brunkhorst,P.Schlattmann, 2013.Procalcitonin as a diagnostic marker for sepsis: a systematic review and meta-analysis,Lancet Infect. Dis 13 426 -35

[24] Watson , R.S.,J.A.Carcillo,Linde-Zwirble , G.,Clermont , J.,Lidicker and D.C.,Angus, 2003.The epidemiology of severe sepsis in children in the United States,AJRCCM 167 695 -701

[25] Zhang , J.P.,Y.Yang,O.Levy,C.Chen, 2010.Human Neonatal Peripheral Blood Leukocytes Demonstrate Pathogen-Specific Coordinate Expression of TLR2, TLR4/MD2 and MyD88 During Bacterial Infection In Vivo,Pediatr. Res 479 -

[26] Zhang , X. D.,Liu , Y.N.,Liu , R.,Wang and L.X.,Xie, 2015.The accuracy of presepsin (sCD14-ST) for the diagnosis of sepsis in adults: a meta-analysis,Crit. Care 323 -

[27] Zhuang , Y.,H.Peng,Y.Chen,S.Zhou,Y.Chen, 2017.Dynamic monitoring of monocyte HLA-DR expression for the diagnosis, prognosis, and prediction of sepsis,Front Biosci 22 -

Citations

Downloads

Views