Abstract:
Wild ediblefruit bearing plants are integral part of the urban communities for nutrition and medicines but its population faces natural and anthropogenic threats. Vitisjacquemontii is a wild grape, locally known as geedarkwar. Some species of Vitis have been explored biotechnologically and pharmaceutically, no or very little scientific studies on V. jacquemontii are described. Objectives of the study were achieved: effective protocols for callus induction and in vitro propagation were developed and phytochemical profile of fruit and leaf extracts was evaluated. Brown and white callus from node explants was induced on media supplemented with Naphthalene Acetic acid (NAA) and Indole-3-acetic acid (IAA) with frequency of 46-64% and 21-29% respectively. Murashige and Skoog (MS) media augmented with Benzyl adenine (BA) most appropriate for multiple shoot induction (72.25%), highest number (8.34) and length (6.4 cm) of shoot at concentrations of 1.5, 2.5 and 2.0 mg/L respectively. MS medium with BA (1.5 mg/L) most suited for shoot initiation (58.52%) and multiplication (6.93) from callus. Similarly, maximum shoot rooting percentage (51.23%) and number of root (5.62) was noted on medium augmented with NAA. The total phenolic contents (TPC), total flavonoid contents (TFC), and total tannin contents (TTC) of V. jacquemontii leaf methanol extract (VJLME) and fruit methanol extract (VJFME) were (101.89, 67.03, 103.20) and 297.65, 92.21, 136.78 mg gallic acid equivalent/g respectively.
Page(s):
299-299
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023