The phytochemical content and antimicrobial activities of Malaysian Calophyllum canum (stem bark). | [Pakistan Journal of Pharmaceutical Sciences • 2012]

Author(s):

1. Suhaib Ibrahim Alkhamaiseh: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang, Malaysia

2. Muhammad Taher: Department of Pharmaceutical Technology, Faculty of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang, Malaysia

3. Farediah Ahmad: Department of Chemistry, Faculty of Science, University Technology Malaysia, Skudai, Johor Malaysia

4. Haitham Qaralleh: Department of Biomedical Science, Faculty of Science, International Islamic University Malaysia, Kuantan, Pahang, Malaysia

5. Osama Yousef Althunibat: Department of Biomedical Science, Faculty of Science, International Islamic University Malaysia, Kuantan, Pahang, Malaysia

6. Deny Susanti: Department of Biomedical Science, Faculty of Science, International Islamic University Malaysia, Kuantan, Pahang, Malaysia

7. Solachuddin Jauhari Arief Ichwan: Faculty of Dentistry, International Islamic University Malaysia, Kuantan, Pahang, Malaysia

Abstract:

Recently there was huge increase in using of ‘herbal products’. These can be defined as plants, parts of plants or extracts from plants that are used for curing disease. However, Calophyllum species is a tropical plant and it has been used in traditional medicine, the limitation in safety and effectiveness information could lead to serious health problems. Providing information for communities by evaluating the phytochemical contents, antioxidant, antimicrobial and cytotoxic activities will improve the therapeutic values. Three main Calophyllum canum fractions (none – high polar) were tested to find out the phenolic, flavonoid, flavonol content, DPPH radical scavenging, reducing power and chelating iron ions. Also were tested against Bacillus cereus, Staphylococcus aureus, Escherichia coli, Psedomonas aeruginosa, Candida albicans, and Cryptococcus neoformans. In addition, cytotoxic activity was assayed against lung cancer A549 cell line. The methanol fraction showed no bioactivity but achieved the highest amount of phenolic, flavonol and flavonoid contents, also it showed a significant result as antioxidant, reducing power and chelating agent. The n-hexane fraction achieved the minimum inhibitory concentration (MIC) value 12.5 µg. mL-1 against B. cereus while the MIC value for DCM fraction was 25 µg. mL-1. The DCM fraction was more active against S. aureus where the result was 50 µg. mL-1 while the n-hexane fraction was 100 µg. mL-1. The three main fractions have shown no activity against gram negative bacterial and fungal. The n-hexane and DCM fractions have shown cytotoxicity against lung cancer cell line; the 50 % inhibition concentration (IC50) was 22 ± 2.64 and 32 ± 3.78 µg. mL-1 respectively. The results were statistically significant (P < 0.05). Among the results, C. canum fractions proved to be effective against gram positive bacterial and anti-proliferation activity. Also it showed antioxidant activity as well. The results provided beneficial information for communities as well as can help to search for alternative drugs, and will contribute to establish safe and effective use of phytomedicines in the treatment of diseases.

Page(s): 555-563

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 25, Issue: 3, Year: 2012

Keywords:

Keywords are not available for this article.

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views