A plant transformation vector containing the gene dehd for the development of cultivars resistant to monochloroacetic acid. | [Journal of Animal and Plant sciences • 2016]

Author(s):

1. E. Mohamed: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, UTM Skudai, Johor, Malaysia

2. F. A. Rahiman: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, UTM Skudai, Johor, Malaysia

3. R. A. Wahab: Faculty of Science, Universiti Teknologi Malaysia, UTM Skudai, Johor, Malaysia

4. C. R. C M. Zain: Faculty of Science and Technology, Universiti Kebangsaan Malaysia (UKM), Bangi, Selangor, Malaysia

5. M. A Javed: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, UTM Skudai, Johor, Malaysia

6. F. Huyop: Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, UTM Skudai, Johor, Malaysia

Abstract:

A dehalogenase D gene (dehD) capable of degrading monochloroacetic acid (MCA) has been previously isolated from Rhizobium sp. RC1 and characterized. The 804-bp dehD gene was inserted into pCAMBIA under the control of the cauliflower mosaic virus 35S promoter and designated pCAMdehD, with a total size of 10,592 bp. The pCAMdehD was introduced into tobacco (Nicotiana benthamiana) via Agrobacterium-mediated transformation. The integration and expression of dehD in N. benthamiana was confirmed by PCR and reverse transcription PCR, respectively. MCAresistant transformants were selected in tissue cultures containing 60 μg/L MCA. Analysis of plants using a leaf-painting assay revealed that transgenic N. benthamiana was resistant to 4.0 G/L MCA compared with 2.0 G/L for non-transformed controls. The use of dehD could thus be advantageous for herbicide-resistant plant breeding systems, and it is also a suitable marker gene for plant-transformation studies. To the best of our knowledge, this is the first report detailing transgenic N. benthamiana engineered with dehD from Rhizobium sp. RC1.

Page(s): 1133-1139

DOI: DOI not available

Published: Journal: Journal of Animal and Plant sciences, Volume: 26, Issue: 4, Year: 2016

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