Tissue culture and RAPD analysis of Cinnamomum camphora and Cinnamomum verum. | [Biotechnology • 2007]

Author(s):

1. J. Govinden Soulange: Department of Agricultural and Food Science, Faculty of Agriculture, The University of Mauritius, Mauritius

2. V. M. Ranghoo-Sanmukhiya: Department of Agricultural and Food Science, Faculty of Agriculture, The University of Mauritius, Mauritius

3. S. D. Seeburrun: Department of Agricultural and Food Science, Faculty of Agriculture, The University of Mauritius, Mauritius

Abstract:

Molecular techniques and tissue culture were used to validate the morphological differences in two species of the Lauraceae family, namely Cinnamomum camphora and Cinnamimum verum. Shoot tip and leaf explants of Cinnamomum camphora and Cinnamomum verum obtained from field grown trees were cultured on MS medium supplemented with various levels of BAP and TDZ. Hundred percent multiple shoot formation was observed in shoot tip explants of C. camphora cultured on MS medium containing 1.0 mg L-1 BAP supplemented with 2.5 mg L-1 TDZ. Leaf explants of C. camphora responded only to MS medium containing 1.0 mg L-1 BAP supplemented with TDZ (0.005-5.0 mg L-1) by forming compact callus. C. verum explants reacted to the treatments by sprouting. All shoots (100%) produced roots two weeks after transfer on the basic MS medium and the plantlets were successfully transplanted to pots containing topsoil plus compost (2:1) after acclimatization. Random Amplified Polymorphic DNA (RAPD) was used as a preliminary technique to evaluate differences between C. camphora and C. verum. All the eleven primers used in this study produced polymorphic bands in both Cinnamomum species. Results are promising and there is scope for developing (Sequence Characterized Amplified Regions) SCAR primers for identification of these two species as well as the other species in the same genus.

Page(s): 239-244

DOI: DOI not available

Published: Journal: Biotechnology, Volume: 6, Issue: 2, Year: 2007

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