Studies on the Physical Parameters for Cephalosporin Biosynthesis from Acremonium chrysogenum by Submerged Fermentation. | [Pakistan Journal of Zoology • 2019]

Author(s):

1. Umar Farooq Gohar: Institute of Industrial Biotechnology, GC University, Lahore-54000, Pakistan

2. Hamid Mukhtar: Institute of Industrial Biotechnology, GC University, Lahore-54000, Pakistan

3. Ali Nawaz: Institute of Industrial Biotechnology, GC University, Lahore-54000, Pakistan

4. Asad Mehmmood: School of Chemistry, University of New South Wales, Australia

Abstract:

The present study was performed to optimize physical parameters for the maximum production of antibiotic (cephalosporin) by isolated fungal strain Acremonium chrysogenum (IIB-10). The strain IIB-10 was cultivated on medium containing (%, w/v): Baker's flour, 1.5; corn meal, 2; Methyl oleate, 1.6; calcium carbonate, 0.3 and ammonium sulphate, 0.1. The cultural conditions such as incubation temperature (30°C), pH (6.5) and inoculum size and age (2%, w/v, 48 h) were optimized. The maximum cephalosporin production was achieved after 144 h of incubation. The dry mycelial mass was found to be 39.46 mg/ml. The antimicrobial activity was assessed by disc diffusion method. Cephalosporin production was estimated by using spectrophotometric technique.

Page(s): 913-918

DOI: 10.17582/journal.pjz/2019.51.3.913.918

Published: Journal: Pakistan Journal of Zoology, Volume: 51, Issue: 3, Year: 2019

Keywords:

Antibiotic , Submerged fermentation , Cephalosporin , Acremonium chrysogenum , Disc diffusion method

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