The sensitivity and specifi city of latex particle agglutination to cerebro spinal fluid culture in etiological diagnosis of bacterial meningitis | [Pakistan Journal of Surgery • 2021]

Author(s):

1. Muhammad Shahid Ghaffar: Hamdard College of Medicine & Dentistry, Hamdard University Hospital, Karachi, Pakistan

2. Abdul Hafeez Smooro: Pakistan Atomic Energy Commission (PAEC) General Hospital, Islamabad, Pakistan

3. Shabeeta Bai: United Medical and Dental College, Karachi, Pakistan

4. Ejaz Ur Rehman: United Medical and Dental College, Karachi, Pakistan

5. Asma Naz: United Medical and Dental College, Karachi, Pakistan

6. Muhammad Saad Raza: United Medical and Dental College, Karachi, Pakistan

Abstract:

Introduction: Bacterial meningitis is an acute and life threatening infection. It present as a medical emergency, which requires rapid diagnosis and immediate treatment to minimize the risk of morbidity and mortality. Unfortunately the presenting signs and symptoms may not be diagnostic and rarely give a clue to the identity of the causative agents. Th e laboratory test like latex particle agglutination test (LPA) is therefore necessary to the diagnosis, and establish the etiological agents. Main outcome measures is sensitivity and specifi city of LPA test was estimated taking CSF culture as standard, result of LPA compared with CSF culture. Objective: To see the sensitivity and specifi city of LPA to cerebro spinal fl uid (CSF) culture in etiological diagnosis of bacterial meningitis particularly when antibiotic treatment has already been started. Design: Cross sectional study. Duration of study: Th e duration of study was one year from 19th August 2019 to 19th August Result: In our study total number of the cases of 42, male to female ratio was 1.5:1. Th e most frequent symptoms were fever, vomiting, poor feeding and seizures. We found the causative organism of bacterial meningitis through LPA in CSF of 10 subjects in spite of no growth in CSF culture. In 2 CSF culture positive bacterial meningitis cases LPA is negative. We found the sensitivity of LPA test in CSF as 80% and specifi city 68.7%. Conclusion: Th e study concludes that latex particle aggultination test is superior test for the etiological diagnosis of bacterial meningitis as compared to the conventional test like CSF culture. It has a high sensitivity and specifi city and is rapid enough to guide the clinician for institution of proper antibiotics. So it should be include as an adjunct laboratory test for establishing the etiological diagnosis of bacterial meningitis particularly in pretreatment cases.

Page(s): 273-277

DOI: DOI not available

Published: Journal: Pakistan Journal of Surgery, Volume: 37, Issue: 4, Year: 2021

Keywords:

cerebrospinal fluid , Bacterial meningitis , Latex particle agglutination test

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