A validated RP-HPLC Method for Determination of Rose Bengal in Bulk and Surgical Strips. | [RADS Journal of Pharmacy & Pharmaceutical Sciences • 2018]

Author(s):

1. Abdul Mannan: Ophth-Pharma (Pvt.) Ltd, Karachi, Pakistan

2. Akhtar Jamal Khan: Ophth-Pharma (Pvt.) Ltd, Karachi, Pakistan

3. Mahwish Khan: Ophth-Pharma (Pvt.) Ltd, Karachi, Pakistan

4. Ghulam Abass: Ophth-Pharma (Pvt.) Ltd, Karachi, Pakistan

5. Sayyad Roohullah: Ophth-Pharma (Pvt.) Ltd, Karachi, Pakistan

6. Zahid Khan: Department of Pharmacognosy, Federal Urdu University of Arts, Science & Technology, Karachi, Pakistan

Abstract:

A simple validated RP-HPLC method for determination of Rose Bengal (RB) Surgical strips which are used for staining of damaged cells of eye. The successful separation of RB was achieved on a Cromosil 100-5µm column (4.6 x 250mm with 5µm diameter) and detector of UV 262nm 1.0ml/min as a flow rate, and 20µl as an injector volume. The mobile phase of proposed method was methanol and phosphate Buffer of pH 8.0 in 50:50 (v/v) ratios. The linearity of method was determined in the range of 8-40ppm. The regression coefficient; LOD and LOQ were respectively 0.9987, 1ppm and 3ppm. It was validated according to the guidelines of ICH and USP.

Page(s): 185-189

DOI: DOI not available

Published: Journal: RADS Journal of Pharmacy & Pharmaceutical Sciences, Volume: 6, Issue: 3, Year: 2018

Keywords:

Validation , Rose Bengal

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