The effect of Dexmedetomidine on Myocardial Ischemia Reperfusion Injury in Streptozotocin Induced Diabetic Rats. | [Anaesthesia, Pain and Intensive Care • 2015]

Author(s):

1. Mustafa Arslan: Department of Anaesthesiology and Reanimation, Gazi University, Ankara, Turkey

2. Fatih Poyraz: Department of Cardiology, Kirikkale University, Afyonkarahisar, Turkey

3. Hasan Ali Kiraz: Department of Anaesthesiology and Reanimation, Onsekiz Mart University, Canakkale, Turkey

4. Metin Alkan: Department of Anaesthesiology and Reanimation, Gazi University, Ankara, Turkey

5. Gulay Kip: Department of Paediatric Dentistry, Medical Faculty, Gazi University, Ankara, Turkey

6. Ozlem Erdem: Department of Pathology, Medical Faculty, Gazi University, Ankara, Turkey

7. Abdullah Ozer: Department of Cardiovascular Surgery Medical Faculty, Kirikkale University, Afyonkarahisar, Turkey

8. Volkan Sivgin: Department of Anaesthesiology and Reanimation, MGazi University, Ankara, Turkey

9. Faruk Metin Comu: Department of Anaesthesiology and Reanimation, Gazi University, Ankara, Turkey

Abstract:

Objective: Ischemia/reperfusion (I/R) injury is an important cause of myocardial damage by means of oxidative, inflammatory, and apoptotic mechanisms. The aim of the present study was to examine the potential cardio-protective effects of dexmedetomidine in a diabetic rat model of myocardial I/R injury. Methodology: A total of 18 streptozotocin (55 mg/kg) induced diabetic Wistar Albino rats were randomly divided into three equal groups as follows: the diabetic I/R group (DIR) in which myocardial I/R was induced by ligating the left anterior descending (LAD) coronary artery for 30 min, followed by 2 hours of reperfusion following left thoracotomy, the diabetic I/R dexmedetomidine group (DIRD) which were given 100 µg/kg dexmedetomidine intraperitoneally 30 min before I/R induction by the same method and the diabetic control group (DC) which underwent sham operations without tightening of the coronary sutures. As a control group (C), 6 healthy age-matched Wistar Albino rats underwent sham operations similar to DC group. After the operation the rats were sacrificied and the myocardial tissues were histopathologically examined. Results: Microscopic myonecrosis findings were significantly different among groups (p=0.008). Myonecrosis findings were significantly higher in DIR compared to C, DC and DIRD groups (p=0.001, p=0.007 and p=0.037 respectively). Similarly microscopic inflammatory cell infiltration degrees showed significant differences among groups (p<0.0001). Compared to C, DC and DIRD groups, the microscopic inflammatory cell infiltration was significantly higher among DIR group (p<0.0001, p<0.0001 and p=0.009 respectively). Also myocardial tissue edema was significantly different among groups (p=0.002). The microscopic myocardial tissue edema levels were significantly higher in DIR group than C and DIRD groups (p<0.0001 and p=0.022 respectively). Tissue edema was also more prominent in DC compared to C group (p=0.022) Conclusion: Taken together our data indicate that dexmedetomidine may be helpful in reducing myocardial necrosis, myocardial inflammation and myocardial tissue edema resulting from ischemia/ reperfusion injury.

Page(s): 444-451

DOI: DOI not available

Published: Journal: Anaesthesia, Pain and Intensive Care, Volume: 19, Issue: 4, Year: 2015

Keywords:

Keywords are not available for this article.

References:

[1] 1996., 334 884 -

[2] .disease in subjects with type 2 diabetes and, -

[3] 1998., 339 229 -

[4] 2007., 154 743 -

[5] Am J Cardiol, 2007., 100 206 -

[6] HORIZONS AMI, 2012.),Am J Cardiol -

[7] .,Apr 10 1111 -6

[8] amjcard., 2011., 11 -

[9] 2007.coronary syndromes,JAMA 298 765 -

[10] 2002., 40 418 -

[11] .Comparison of factors associated with 30-, -

[12] 1998.,Cardiol 81 7 -

[13] 2012.ischemic preconditioning in patients with type 1 diabetes mellitus: a pilot study,Cardiovas Diabetol 10 1475 -

[14] EngelhardK,WernerC,EberspächerE,BachlM,BlobnerM,HildtE, 2003.The effect of the alpha 2-agonist dexmedetomidine and the N-methyl-D-aspartate antagonist S(+)- ketamine on the expression of apoptosisregulating proteins after incomplete cerebral ischemia and reperfusion in rats,Anesth Analg 96 524 -

[15] DahmaniS,RouelleD,GressensP,MantsJ., 2005.Effects of dexmedetomidine on hippocampal focal adhesion kinase tyrosine phosphorylation in physiologic and ischemic conditions,Anesthesiology 103 969 -

[16] OkadaH,KuritaT,MochizukiT,MoritaK,SatoS., 2007.The cardioprotective effect of dexmedetomidine on global ischemia in isolated rat hearts,Resuscitation 74 538 -

[17] YoshitomiO,ChoS,HaraT,ShibataI,MaekawaT,UreshinoH, 2012.Direct protective effects of dexmedetomidine against myocardial ischemiareperfusion injury in anesthetized pigs,Shock 10 92 -

[18] CanM,GulS,BektasS,HanciV,AcikgozS., 2009.Effects of dexmedetomidine or methylprednisolone on inflammatory responses in spinal cord injury,doi: 1 0 . 1 1 1 1 / j . 1 3 9 9 - 6 5 7 6 . 2 0 0 9 . 0 2 0 1 9 1068 -

[19] LaiYC,TsaiPS,HuangCJ, 2009.Effects of dexmedetomidine on regulating endotoxininduced up-regulation of inflammatory molecules in murine macrophages,J Surg Res 154 212 -

[20] TüreciE,İşM,ÜzümG,AkyüzF,UluMO,DöşoğluM, 2009.Alterations in bloodbrain barrier after traumatic brain injury in streptozotocin-induced diabetic rats,J Nervous Sys Surgery 2 79 -

[21] CakirM,PolatA,TekinS,VardiN,TaslidereE,Z, 2015.The effect of dexmedetomidine against oxidative and tubular damage induced by renal ischemia reperfusion inrats,Ren Fail 1011550 1 -

[22] WangH,ChenH,WangL,LiuL,WangM,LiuX, 2014.Acute hyperglycemia prevents dexmedetomidine-induced preconditioning against renal ischemia-reperfusion injury,Acta Cir Bras 10 812 -8

[23] JeonYT,HwangJW,LimYJ,ParkSK,ParkHP, 2013.Postischemic sevoflurane offers no additional neuroprotective benefit to preischemic dexmedetomidine,J Neurosurg Anesthesiol 10 184 -90

[24] KocogluH,KaraaslanK,GoncaE,BozdoganO,GulcuN., 2008.Preconditioning effects of dexmedetomidine on myocardial ischemia/reperfusion injury in rats,Curr Ther Res Clin Exp 69 150 -

[25] LohHK,SahooKC,KishoreK,RayR,NagTC,KumariS, 2007.Effects of thalidomide on isoprenaline-induced acute myocardial injury: a haemodynamic, histopathological and ultrastructural study,Basic Clin Pharmacol Toxicol 100 233 -

[26] BrownleeM., 2005.The pathobiology of diabetic complications: A unifying mechanism,Diabetes 54 1615 -

[27] CerielloA, 2005.Acute hyperglycaemia: A “new” risk factor during myocardial infarction,Eur Heart J 26 328 -

[28] FrustaciA,KajsturaJ,ChimentiC,JakoniukI,LeriA,MaseriA, 2000.Myocardial cell death in human diabetes,Circ Res 87 1123 -

[29] FiordalisoF,LiB,LatiniR,SonnenblickEH,SonnenblickEH,AnversaP, 2000.Myocyte death in streptozotocin-induced diabetes in rats in angiotensin II-dependent,Lab Invest 80 513 -

[30] MarfellaR,D'AmicoM,Di FilippoC,PiegariE,NappoF,EspositoK, 2002.Myocardial infarction in diabetic rats: role of hyperglycaemia on infarct size and early expression of hypoxia-inducible factor 1,Diabetologia 45 1172 -

[31] TsaoPS,AokiN,LeferDJ,JohnsonIII G,LeferAM, 1990.Time course of endothelial dysfunction and myocardial injury during myocardial ischemia and reperfusion in the cat,Circulation 82 1402 -

[32] GottliebRA,BurlesonKO,KlonerRA,BabiorBM,EnglerRL, 1994.Reperfusion injury induces apoptosis in rabbit cardiomyocytes,J Clin Invest 94 1621 -

[33] SongQ,AnX,LiD,SodhaNR,BoodhwaniM,TianY, 2009.Hyperglycemia attenuates angiogenic capability of survivin in endothelial cells,Microvasc Res 78 257 -

[34] AlegriaJR,MillerTD,GibbonsRJ,YiQL,YusufS., 2007.Infarct size, ejection fraction, and mortality in diabetic patients with acute myocardial infarction treated with thrombolytic therapy,Am Heart J 154 743 -

[35] QiaoH,SandersRD,MaD,WuX,MazeM., 2009.Sedation improves early outcome in severely septic Sprague Dawley rats,Crit Care 13 R136 -

[36] TaniguchiT,KidaniY,KanakuraH,TakemotoY,YamamotoK., 2004.Effects of dexmedetomidine on mortality rate and inflammatory responses to endotoxininduced shock in rats,Crit Care Med 32 1322 -

[37] VennRM,BryantA,HallGM,GroundsRM, 2001.Effects of dexmedetomidine on adrenocortical function, and the cardiovascular, endocrine and inflammatory responses in post-operative patients needing sedation in the intensive care unit,Br J Anaesth 86 650 -

[38] MemisD,HekimogluS,VatanI,YandimT,YükselM,SütN., 2007.Effects of midazolam and dexmedetomidine on inflammatory responses and gastric intramucosal pH to sepsis, in critically ill patients,Br J Anaesth 98 550 -

[39] PengM,WangYL,WangCY,ChenC, 2013.Dexmedetomidine attenuates lipopolysaccharide-induced proinflammatory response in primary microglia,J Surg Res 179 e219 -

[40] SchömigA,RichardtG., 1990.The role of catecholamines in ischemia,J Cardiovasc Pharmacol 16 105 -

[41] LamerisTW,de ZeeuwS,AlbertsG,BoomsmaF,DunckerDJ,VerdouwPD, 2000.Time course and mechanism of myocardial catecholamine release during transient ischemia in vivo,Circulation 101 2645 -

[42] RoekaertsPM,PrinzenFW,LangeSD, 1996.Beneficial effects of dexmedetomidine on ischemic myocardium of anaesthetized dogs,Br J Anaesth 77 427 -

[43] Matko,Fehe´,ViziES, 1994.Receptor mediated presynaptic modulation of the release of noradrenaline in human papillary muscle,Cardiovasc Res 28 700 -

[44] HoffmanWE,KochsE,WernerC,ThomasC,AlbrechtRF, 1991.Reversal by the alpha 2-adrenergic antagonist atipamezole,Anesthesiology 75 328 -

[45] MaierC,SteinbergGK,SunGH,ZhiGT,MazeM., 1993.Neuroprotection by the alpha 2-adrenoreceptor agonist dexmedetomidine in a focal model of cerebral ischemia,Anesthesiology 79 306 -

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