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Transfer of the GAFP and NPI, two disease-resistant genes, into a Phalaenopsis by Agrobacterium tumefaciens.
Author(s):
1. Jie Li: College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
2. Ping Kuang: College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
3. Ren-Dao Liu: College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
4. Dan Wang: College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
5. Zhi-Na Wang: College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China
6. Min-Ren Huang: Laboratory of Forest Genetics and Gene Engineering, Nanjing Forestry University, Nanjing 210037, China
Abstract:
Gastrodia Antifungal Protein (GAFP) and Neutrophils Peptide-I (NPI), two disease-resistant genes were successfully transferred into the Dtps.Tailin Angel Phalaenopsis by using Agrobacterium strain off gradual methods. Two hundred and eighty kanamycin-resistant plants were regenerated by this method. PCR, Southern blot and RT-PCR confirmed that GAFP and NPI genes have been integrated into the genome of Phalaenopsis. In vitro antibiosis assay of the Colletotrichum gloeosporioides Sacc suggested that the transgenic plants were disease-resistant. Disease-resistance experiments proved that both GAFP and NPI genes were expressed efficiently in Phalaenopsis.
Page(s): 1761-1766
DOI: DOI not available
Published: Journal: Pakistan Journal of Botany, Volume: 45, Issue: 5, Year: 2013
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