GC-MS analysis, phytochemical profile and evaluation of antioxidant and toxicity property of aerial parts of haplophyllum gilesii | [Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23 • 2023]

Author(s):

1. Saleha Ashfaq: Dept. of Biology, The University of Haripur, Haripur, KP, Pakistan

2. Manzoor Hussain: Dept. of Botany,Hazara University Mansehra, Mansehra, Pakistan

3. Aziz Ullah: Dept. of Biology, The University of Haripur, Haripur, KP, Pakistan

4. Asma Ul Husna: Dept. of Biology, The University of Haripur, Haripur, KP, Pakistan

5. Wajeeha Saeed: Dept. of Biology, The University of Haripur, Haripur, KP, Pakistan

6. Nazish Bibi: Dept. of Microbiology, CAE Hazara UniversityMansehra, Mansehra, Pakistan

7. Muhammad Junaid: Dept. of Microbiology, CAE Hazara UniversityMansehra, Mansehra, Pakistan

Abstract:

Haplophyllum gilesii is an endemic plant species reported from Northern Pakistan, belonging to family Rutaceae. It chiefly contains volatile oils, alkaloids, fixed oils, phenolic compounds and furanocoumarins, well known for ethno-medicinal applications. In the present study the essential oil of the aerial parts of Haplophyllumgilesii was carried by hydrodistillation. 32 phytochemical compounds were identified by GC-MS analysis. Further, aerial parts of H. gilesii in methanol extract and its soluble fractions i-en-hexane, ethyl acetate, and chloroform were screened for in vitro antioxidant activity by 2,2-diphenyl-1- picrylhydrazyl (DPPH) assay and Ferric Reducing Antioxidant Power (FRAP) assay using potassium ferrocyanide ferric chloride methods. Ascorbic acid was used as standard. The total phenolic, total flavonoid, and extraction yield of the respective solvents were also estimated to access the correlation with the antioxidant potential of the extracts. The total phenolic content of the aerial parts of H. gilesiiwas determined by gallic acid equivalent whereas, total flavonoid content was determined by rutin equivalent using the colorimetric method respectively. Methanol extract showed a maximum antioxidant activity with an IC50 value of 51.27±0.39µg/mL while ethylacetate fraction exhibited minimum antioxidant potential with an IC50value of 216 ± 0.41 µg/mL. Similarly, among all the extracts evaluated the highest concentration of total phenolic, total flavonoid content, and extraction yield was detected in methanol extract. Moreover, our results of toxicology demonstrated that the aqueous extract did not show any toxic effects on all the tested groups. No mortality and significant weight loss were observe data dose of 2000mg/kg and 5000mg/kg in the plant extract treated groups compared to the control group in acute and sub-acute toxicity assay. To best of our knowledge this is the first systematic study on phytochemical screening of Haplophyllum gilesii. Results of our study revealed that phytochemical compounds identified might have maximum antioxidant potential and this research could open new horizons for discovery of the novel drugs for various cancer cell lines.

Page(s): 194-194

DOI: DOI not available

Published: Journal: Abstract Book on International Conference on Food and Applied Sciences (ICFAS-23) 3-5 August 23, Volume: 0, Issue: 0, Year: 2023

Keywords:

essential oil , DPPH , GCMS , antioxidant , Flavonoids , Phenolics , toxicity , Haplophyllumgilesii

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