Author(s):
1. Zaryab Khalid Sial:
Department of Botany, LCWU, Lahore, Pakistan
2. Kashmala:
Department of Botany, LCWU, Lahore, Pakistan
3. Shabnum Shaheen:
Department of Botany, LCWU, Lahore, Pakistan
4. Riffat Siddique Sana Khalid:
Department of Botany, LCWU, Lahore, Pakistan
Abstract:
The viroids are the smallest living infectious agents without any protein coat that are thought to only infect plants. Nucleic acid (DNA) transfer between plants is a natural phenomenon which is likely to occur in many ways. Apple skin scar viroid has broad herbaceous range of host plants. The active transmission of Apple scar skin viroid (ASSVd) and naked ssRNA species by the whitefly Trialeurodes vaporariorum (Tv) was confirmed during present study in cucumis sativis. It was matter of great interest that not only the viroidal RNAs, its DNA type was also identified from the targeted insect. The viroid transfer efficiency was enhanced with the help of Cucumis sativus Phloem protein 2 (CsPP2), This protien/ASSVd complex was stably present in the viroid infected cucumber plants, as was identified with the help of immunological Assay.As viroid-like secondary structures are found in some plant RNAs, and PP2 is known to bind and translocate several RNAs, the results have huge implications in transfer of these RNA species between plants visited by the whitefly. To our knowledge, there are no confirmed reports that demonstrate the use of host molecular markers for the purpose of detecting generic plant viroidal infection.
Page(s):
188-188
DOI:
DOI not available
Published:
Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023
Keywords:
Cucumis sativus
,
Apple skin scar viroid
,
hloem Protein 2
,
ssRNA
,
Nucleic acid DNA
References:
References are not available for this document.
Citations
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