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a-Amylase production by Bacillus licheniformis under solid state fermentation conditions and its cross linking with metalosalts to confer thermostability.
Author(s)
Mubashir Niaz Laboratory of Mycology and Biotechnology, Department of Botany, GC University, Faisalabad, Pakistan
Tehreema Iftikhar Laboratory of Mycology and Biotechnology, Department of Botany, GC University, Faisalabad, Pakistan
Humaira Saleem Laboratory of Mycology and Biotechnology, Department of Botany, GC University, Faisalabad, Pakistan
Syed Qaiser Abbas Laboratory of Mycology and Biotechnology, Department of Botany, GC University, Faisalabad, Pakistan
Romana Tabassum Industrial Biotechnology Division, National Institute for biotechnology and Genetic Engineering, Faisalabad, Pakistan
Muhammad Anjum Zia Enzyme Biotechnology Lab, Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad, Pakistan
Ikram U. Haq Institute of Industrial Biotechnology, GC University, Lahore, Pakistan
Abstract
The production potential of Bacillus licheniformis strain RT7YC for extracellular α-amylase was analyzed through solid state fermentation. Enzyme concentration produced under varying concentrations of substrate and ecological variables was calculated by using DNS and starch-iodine methods. The maximum production of α-amylase was obtained at 20% wheat bran. It was observed that corn steep liquor was found as best nitrogen source for α-amylase production. It was also found that enzyme was stable at 60oC when treated for 10 min. Moreover, it was also much stable at 10 mM concentration of CaCl2.
Publication Details
Page(s) 793-795
DOI DOI not available
Published Journal: International Journal of Agriculture and Biology, Volume: 12, Issue: 5, Year: 2010
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