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1-Variation in cell morphology and gram-staining property of Bacilli under different salt stresses and media composition.
Author(s):
1. Erum Shuaib: Botany Department, University of the Punjab, Quaid-a-Azam Campus, Lahore¬54590, Pakistan
2. Uqba Mehmood: Botany Department, University of the Punjab, Quaid-a-Azam Campus, Lahore¬54590, Pakistan
3. Shahida Hasnain: Botany Department, University of the Punjab, Quaid-a-Azam Campus, Lahore¬54590, Pakistan
Abstract:
Hs-3, Hs-4 and Az-9 are salt tolerant strain, which show Gram negative to Gram-variable staining behavior under varying environmental conditions. These strains were grown in different media composition (lowry, nutrient, pennasy and M-9 minimal both in broth cultures and agar media) and salt stresses (MgSO4, KCL, KNO3) supplemtentated with 0.1 and 1 M of NaCl at 37°C for 4, 8, 16 and 24 hours. Media composition and various salts stress manifested great variation in straining behavior and cell morphology. Az-9 exhibited maximum variation in staining and morphology in rich medium. Hs-3 showed maximum filamentation under KCL stress in pennassy medium. KCL and KNO3 stresses caused filamentation, in all strains while spore formation was pronounced under MgSO4 and NaCl stress in Az-9 in nutrient agar. Pottassium salt caused adverse affects on cell morphology by degeneration or lysis of cells with passage of time.
Page(s): 129-133
DOI: DOI not available
Published: Journal: Science International, Volume: 16, Issue: 2, Year: 2004
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