Alternative Culture Media for Growth and Sporulation of Trichodermaharzianum. | [Pakistan Journal of Biotechnology • 2017]

Author(s):

1. Liqaa Y Mohsen: Department of Biology, College of Science, University of Babylon, Iraq

2. Haider J Kadhim: University Performance Evaluation Unit, Al-Mustaqbal University College

3. Jawad K Abood Aljanabi: Department of Biology, College of Science, University of Babylon, Iraq

4. A.N Al-Yassiry: Department of Biology, College of Science, University of Babylon, Iraq

Abstract:

Microorganisms need nutrients for their biological activities and reproduction. Culture media such PDA, used for growing fungi in laboratories. Because of high cost and don't available at all time. The present study was conducted to supersede Potato in PDA medium with powdered leaves of either Moringa oleifera or mint and to examine their effect on mycelial, conidial and biomass production of bioagent fungus Trichoderma harzianum and pathogenic fungi: Fusarium graminearum and F. oxysporum. The patterns of T. harzainum growth was substantially affected by Moringa Dextrose Agar (MoDA) and Mint Dextrose Agar (MiDA). Both the media were found to magnify the mycelial growth of bioagent fungus as compared to pathogenic fungus F. oxysporum. MoDA characterized by enhancing spore production in T. harzainum (19.6×108), in comparison to Fusarium graminearum (7.13×108) and F. oxysporum (8.73×108) after 9 days of inoculation. Dry weights of T. harzainum (0.75 mg) and F. oxysporum (0.35 mg) mycelia were also sharply incremented in MoDB compared to PDB (0.2 and 0.15gm) respectively in which the dry weight not inhibited by modified media. It was concluded that the utilization of M. oleifera or Mint leaves as a component of culture media in laboratories is a feasible and cheap source as compared to commercially prepared PDA. Furthermore, the present formulated media could be subsidiary act as a selective medium for the magnification and sporulation of T. harzianum. In integration, the leaf powders of both sources can be stored for longer time periods in comparison to potato.

Page(s): 587-593

DOI: DOI not available

Published: Journal: Pakistan Journal of Biotechnology, Volume: 14, Issue: 4, Year: 2017

Keywords:

Keywords are not available for this article.

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