Antiviral, Embryo Toxic and Cytotoxic Activities of Astragalus membranaceus Root Extracts. | [Pakistan Journal of Pharmaceutical Sciences • 2019]

Author(s):

1. Humaira Majeed Khan: Institute of Pharmacy, Lahore College for Women University, Lahore, Pakistan

2. Syeda Maryam Raza: Institute of Pharmacy, Lahore College for Women University, Lahore, Pakistan

3. Aftab Ahmad Anjum: Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan

4. Muhammad Asad Ali: Department of Microbiology, University of Veterinary and Animal Sciences, Lahore, Pakistan

5. Hamid Akbar: Department of Clinical Medicine and Surgery, University of Veterinary and Animal Sciences, Lahore, Pakistan

Abstract:

Antiviral activity of Astragalus membranaceus aqueous and methanol root extracts was determined against Avian influenza H9 virus. Toxicity profile of extracts was evaluated using chicken embryos and BHK-21 cell line. Different concentrations (400, 200, 100, 50, 25. 12.5, 6.25 and 3.12µg/mL) of both aqueous and methanol extracts were mixed with standard virus inoculum (4HAunits) and incubated for 30minutes at 37oC prior to inject the chicken embryos. Chorioallantoic fluid harvested 72 hours post inoculation and evaluated for virus growth using hemagglutination assay. Same concentrations of both extracts without virus were injected in chicken embryos to evaluate embryo toxic activity as well. The cytotoxic activity of aqueous and methanol extracts was determined by MTT colorimetric assay using BHK-21 cells. Three concentrations (400, 200 and 100µg/mL) of aqueous and five concentrations (400, 200, 100, 50 and 25µg/mL) of methanol extract showed antiviral activity. None of the tested concentrations of aqueous and methanol A. membranaceus root extracts caused chicken embryo mortality. Cell survival percentage of aqueous extract was higher than 50 at all of the tested concentrations except 400µg/mL. Two concentrations (400 and 200µg/mL) of methanol extract showed cytotoxicity. It was concluded that aqueous and methanol roots extracts of A. membranaceus have antiviral activity and concentrations which were safe may be used for treatment of Avian influenza H9 virus infections.

Page(s): 137-142

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 32, Issue: 1, Year: 2019

Keywords:

avian influenza H9 virus

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