Adipogenic gene expression and regulation pattern in adipose tissues located on mammary, inguinal and renal fat pad of Korean black goat (Capra hircus). | [Pakistan Veterinary Journal • 2015]

Author(s):

1. S Subi: Department of Animal Biotechnology, College of Animal life sciences, Kangwon National University, Republic of Korea

2. N K Singh: Department of Animal Biotechnology, College of Animal life sciences, Kangwon National University, Republic of Korea

3. A M M T Reza: Department of Animal Biotechnology, College of Animal life sciences, Kangwon National University, Republic of Korea

4. S Shiwani: Department of Animal Biotechnology, College of Animal life sciences, Kangwon National University, Republic of Korea

5. J D Lohakare: Department of Animal Biotechnology, College of Animal life sciences, Kangwon National University, Republic of Korea

6. D K Jeong: Department of Animal Biotechnology, College of Animal life sciences, Kangwon National University, Republic of Korea

7. S J Lee: Department of Animal Biotechnology, College of Animal life sciences, Kangwon National University, Republic of Korea

Abstract:

Development of new fat cells appears to be a permanent event throughout the life process of both humans and animals. Though, this process appears to be similar all over the body, but whether their anatomical location keeps them distinct in their genetic makeup is quite uncertain. Therefore, to investigate the difference in the adipogenic gene expression and their regulation pattern at the molecular level, we collected adipose tissues surgically from prepubertal Korean black goat reared in stall fed system and with high fat rich diet. Perirenal fat, inguinal fat and mammary fat pad adipose tissues were collected and cyropreserved in LN2 for mRNA isolation subsequently. Reverse transcriptase PCR revealed significant (P

Page(s): 48-52

DOI: DOI not available

Published: Journal: Pakistan Veterinary Journal, Volume: 35, Issue: 1, Year: 2015

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