A lytic Podophage Specific to Fish Pathogenic Edwardsiella tarda. | [Pakistan Journal of Biotechnology • 2018]

Author(s):

1. Parichat Phumkhachorn: Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Warin Chamrap, Ubon Ratchathani 34190, Thailand

2. Pongsak Rattanachaikunsopon: Department of Biological Science, Faculty of Science, Ubon Ratchathani University, Warin Chamrap, Ubon Ratchathani 34190, Thailand

Abstract:

A lytic bacteriophage specific to Edwardsiella tarda, a fish pathogen, was isolated from fish culturing pond water. The bacteriophage, designated P8, was highly specific to the species E. tarda. It was tolerant to high temperature up to 70°C and to pH from 4 to 11. As studied by transmission electron microscope, the bacteriophage was a short-tail bacteriophage. The genome of bacteriophage P8 was found to be double stranded DNA, indicated by its sensitivity to EcoRI. Based on its morphology and genome, bacteriophage P8 was classified as a podophage. Five major proteins of bacteriophage P8 (70, 45, 35, 37 and 23 kDa) were detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis. This study shows that bacteriophage P8 had specificity, host range and stability favoring its use as a therapeutic agent against E. tarda infection in aquaculture.

Page(s): 117-121

DOI: DOI not available

Published: Journal: Pakistan Journal of Biotechnology, Volume: 15, Issue: 1, Year: 2018

Keywords:

Keywords are not available for this article.

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