Inferring protein expression alteration instigated by AlCl3/D-galactose induced oxidative stress | [Abstract Book on 9th Annual Neuroscience Conference (ANC-23) August 12-13, 2023 • 2023]

Author(s):

1. Beena Hasan: Department of Biochemistry, University of Karachi, Karachi, Pakistan

2. Saba Zehra: Department of Biochemistry, University of Karachi, Karachi, Pakistan

3. Sidra Waheed: Department of Biochemistry, University of Karachi, Karachi, Pakistan

4. Hira Ali: Department of Biochemistry, University of Karachi, Karachi, Pakistan

5. Sana Farooq:

Abstract:

AlCl3 and D-galactose conventionally used to produce neurodegeneration give rise to effects mimicking Alzheimer’s disease like cognitive impairments. It also induces oxidative stress which further aggravate the tissue damage and associated complications. Proteins are highly vulnerable to this increased oxidative stress and displays alteration in expression and function consequently unbalancing the normal physiology. The present study aims to investigate the effect of oxidative stress and AlCl3 /D-galactose induced neurodegeneration on protein expression in animal models. Two groups of Albino Wistar rats were taken as Test and Control. The test group were intraperitoneally injected with AlCl3 and D-galactose simultaneously at a dose of 200mg/kg and 150mg/kg respectively only for one week followed by behavioral study using Open Field Test, Elevated Plus Maze, Forced Swim Test and Morris Water Maze. After decapitation Catalase, Glutathione and TBARS levels were measured in both groups to estimate the oxidative stress. Protein expression analysis using SDS-PAGE was performed to analyze the expression alteration among the two groups. The test group reveals behavioral deficits including elevated anxiety and depression levels and decrease locomotor activity along with decrease exploratory activity in comparison with control group. Similarly oxidative stress markers were also found to be increased in the test animals. Moreover SDS-PAGE analysis exhibited quantitative differences between control and test samples of rats. This profound biochemical analysis renders a framework which can commence up with new opportunities to provide insights of differential protein expression as a result of AlCl3/ D-galactose induced oxidative stress and neurodegeneration.

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Published: Journal: Abstract Book on 9th Annual Neuroscience Conference (ANC-23) August 12-13, 2023 , Volume: 0, Issue: 0, Year: 2023

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