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A UV-Vis spectrophotometric method for the calculation of aldehyde content in polysaccharides by using 2,3,5-triphenyltetrazolium chloride.
Author(s):
1. Afzal Ahmad: Key Laboratory of Science and Technology of Eco-textile, Ministry of Education, Jiangnan University, Lihu Ave, Wuxi, Jiangsu, China
2. Qiang Wang: Key Laboratory of Science and Technology of Eco-textile, Ministry of Education, Jiangnan University, Lihu Ave, Wuxi, Jiangsu, China
Abstract:
In this study, a simple technique was developed to determine the aldehyde content on an oxidized-polysaccharide. 2,3,5-triphenyltetrazolium chloride (TTC) was used to measure aldehyde analytically by UV-Vis spectrophotometric assay. Comparable results were obtained when tested against the known aldehyde contents of formaldehyde, acetaldehyde, and glutaraldehyde. The TTC UV-Vis assay was further employed to check the aldehyde produced during oxidation of natural polysaccharide konjac glucomannan (KGM). It was found that TTC UV-Vis assay was accurate even at low concentrations of aldehyde and thus this assay could be used to optimize the reaction parameters of oxidation.
Page(s): 101-105
DOI: DOI not available
Published: Journal: Science International, Volume: 31, Issue: 1A, Year: 2019
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