Abstract:
Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy, accounting for nearly 96% of thyroid cancers worldwide, and showing the prevalence of 73.7% in Pakistan, predominantly affecting middle-aged women. Despite improvements in diagnostic methods, the molecular mechanisms contributing to PTC progression remain incompletely defined. This study aimed to evaluate the expression of microRNA-1205 and the transcription factor E2F3, and to explore their association with clinical features of PTC. Fifty patients with histologically confirmed PTC were recruited from hospitals in Rawalpindi and Islamabad after obtaining ethical approval. Paired tumor and adjacent normal thyroid tissues were collected alongside demographic and clinical data, including age, gender, tumor size, stage and thyroid hormone levels. TSH, T3 and T4. RNA was extracted, reverse-transcribed into cDNA and analyzed using real-time PCR. Expression levels of miR-1205 and E2F3 were quantified with GAPDH as reference. Statistical correlations with clinical parameters were assessed using SPSS software. Both miR-1205 and E2F3 were upregulated in PTC tissues compared to adjacent controls. Mean fold changes were 1.0 vs 0 .7 for miR-1205 and 45 vs 10 for E2F3. However, differences were not statistically significant. (P > 0.05). Correlation analysis revealed weak known significant associations between miR-1205 and age, tumor size, and thyroid hormone levels. Similar non-significant correlations were observed for E2F3. A weak positive trend was noted between miR-1205 and E2F3 expression. The study highlighted measurable upregulation of miR-1205 and E2F3 in PTC, suggesting potential oncogenic roles. Although statistical significance was lacking, the findings support further investigation of these markers in larger cohorts and functional studies to clarify their roles in thyroid tumorigenesis and their value as potential biomarkers.
Page(s):
228-228
DOI:
DOI not available
Published:
Journal: 4th International Conference of Sciences “Revamped Scientific Outlook of 21st Century, 2025” , November 12,2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
Gene expression
,
Biomarker
,
Papillary thyroid carcinoma
,
miR1205
,
E2F3