The Complete Mitochondrial Genome Sequence and Phylogenetic Analysis of Squalidus nitens (Cypriniformes: Cyprinidae: Gobioninae) | [Pakistan Journal of Zoology • 2025]

Author(s):

1. Yushuang Zhao: College of Life Science, Southwest Branch of the National Freshwater Fishery Engineering Technology Research Center, China West Normal University,Nanchong 637000,China

2. Jin Li: College of Life Science, Southwest Branch of the National Freshwater Fishery Engineering Technology Research Center, China West Normal University,Nanchong 637000,China

3. Ling Mao: College of Life Science, Southwest Branch of the National Freshwater Fishery Engineering Technology Research Center, China West Normal University,Nanchong 637000,China

4. Juan Guo: College of Life Science, Southwest Branch of the National Freshwater Fishery Engineering Technology Research Center, China West Normal University,Nanchong 637000,China

5. Yu Zeng: College of Life Science, Southwest Branch of the National Freshwater Fishery Engineering Technology Research Center, China West Normal University,Nanchong 637000,China

Abstract:

Squalidus nitens is a common small-bodied freshwater fish endemic to the middle and lower reaches of the Yangtze River. Genetic information on the species is quite scarce, as well as scientists have long disagreed on how to dispose of Squalidus and its related genera. In this study, we primarily reported the complete mitochondrial DNA genome of S. nitens by high-throughput sequencing, and explored the phylogenetic position of S. nitens within the subfamily Gobioninae. The entire length of the mitochondrial genome is 16,606 bp, containing 13 protein-coding genes (PCGs), 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and a control region (D-loop). The nucleotide composition was made up of 29.97% A, 27.26% C, 16.93% G, and 25.84% T, respectively, indicating an A + T (55.81%)-rich feature in the S. nitens. Squalidus shares close skinship with Hemibarbus, and both were found at the base of the evolutionary tree of subfamily Gobioninae by creating sister groups in a well-supported way, according to ML and BI analyses. The closest relatives to S. nitens are S. chankaensis and S. gracilis, not S. argentatus.

Page(s): 337-342

DOI: 10.17582/journal.pjz/20220510150556

Published: Journal: Pakistan Journal of Zoology, Volume: 57, Issue: 1, Year: 2025

Keywords:

phylogenetic relationship , mitochondrial genome , Cyprinidae , Gobioninae , Squalidus nitens

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