Role of Hydrogen Peroxide (H2O2 ) and Trichoderma koningii in Reducing Root Rot Disease of Tomato Caused by Fusarium solani | [Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences • 2024]

Author(s):

1. Qusai H. Madhi: Department of Plant Protection, College of Agriculture, University of Misan,,Iraq

2. Yehya A. Salih: Department of Plant Protection, College of Agriculture, University of Basrah,,Iraq

3. Ghassan M. Daghir: Department of Plant Protection, College of Agriculture, University of Misan,,Iraq

Abstract:

Two isolates of the pathogenic fungus Fusarium solani the causative of tomato root rot disease named Fs1 and Fs2 were isolated from tomato plants infected with root rot disease. Results showed that both examined isolates had significant efects on the percentage of seed germination of tomato and damping-of disease. Thus, isolate Fs1 was more efective than isolate Fs2, as it recorded a percentage of germination and damping-of 56.6 and 71.6% respectively, compared to the control treatment, which recorded 100 and 0% respectively. It was found that the use of hydrogen peroxide (H2O2) at concentrations of 50, 100 and 200 ppm had a significant efect on the fungal radial growth, dry weight (DW) and sporulation of F. solani. Antifungal activity of H O appeared even at the lowest concentration 2 2 (50 ppm), which inhibited radial growth to 49.33%, and decreased the dry weight to 498 mg, and the sporulation to 3.75×106 spores. Additionally, the results indicated significant inhibitory efects of hydrogen peroxide (H 2O2) and the bioagent Trichoderma koningii on F. solani growth. It was noticed that H2O2 has compatible efects with T. koningii, where the antagonistic ability of T. koningii against F. solani increased when the concentration of H2O2 had increased. The results revealed that the treatment F. solani + H O2 (200 ppm) + T. koningii significantly reduced the percentage of 2 damping-of and plant survival to 9.40 and 5.39% respectively, in comparison with the control treatment and F. solani alone treatment, which reached 20.22, 14.48, 34.32 and 67.41% respectively.

Page(s): 171-179

DOI: 10.53560/PPASB(61-2)1023

Published: Journal: Proceedings of the Pakistan Academy of Sciences: B. Life and Environmental Sciences, Volume: 61, Issue: 2, Year: 2024

Keywords:

Biological Control , Solanum lycopersicum L , Dampingof

References:

[1] M.A.R. Alvarenga . .Tomate produção em campo. 2nd edn., : .

[2] Universidade Federal de Lavras ,Lavras .2013 .. , : .

[3] Kapoor. .2004 .Antioxidants in tomato (Lycopersium esculentum) as a function of genotype. Food Chemistry, 84 : 45-51.

[4] Crops FAOSTAT.,Products Livestock .2021 .Food and Agriculture Organization of the United Nations (. , : .

[5] Statistical Organization Central,Three Central .2021 .(In Arabic). , : .

[6] Gonzalez Y.S.,Dijkxhoorn Y.,Elings A.,I. Koomen A.,Obeng P. .2014 .Vegetables business opportunities in Ghana. Wageningen UR: Wageningen, The Netherlands (. , : .

[7] Shenashen M.,Derbalah A.,Mohamed A.,Tuzun S.,Safty S. El .2017 .Recent trend in controlling root rot disease of tomato caused by Fusarium solani using alumina silica nanoparticles. International Journal of Advanced Research in Biological Sciences, 4(6) : 105-119.

[8] Gaigole A.H.,Wagh G.N.,Khadse A.C. .2011 .Antifungal activity of Trichoderma species against soil borne pathogens. Asiatic Journal Biotech Resources, 461(4) : .

[9] Harman G.E.,Howell C.R.,Viterbo A.,I. Chet A.,Lorito M. .2004 .Trichoderma species-opportunistic, avirulent plant symbionts. Natural Reviews Microbiology, 2 : 43-56.

[10] Waghunde R.R.,Shelake R.M.,A R.M.,Sabalpara N. .2016 .Trichoderma: A significant fungus for agriculture and environment. African Journal of Agricultural Research, 1(22) : 1952-1965.

[11] Zaghloul R.A.,Ehsan A.,Hanafy N.,Neweigy A.,Neamat A.K. .2007 .Application of biofertilization and biological control for tomato production. , 18 : 198-212.

[12] Salih Y.A.,Al-Mansoury B.A. .2021 .Evaluation of the eficiency of some bioagents and their interaction with the fungicide topsin-m in the controlling eggplant root rot disease caused by Fusarium oxysporum. International Journal of Agricultural and Statistical Sciences, 17(Supplement 1) : 2153-2162.

[13] Al-Abbas H.H.,Salih Y.A. .2022 .Evaluation of the eficiency of the bioagent Trichoderma longibrachiatum against root rot disease of cucumber plant caused by the fungus Fusarium solani. International Journal of Agricultural and Statistical Sciences, 18(Supplement 1) : 1395-1401.

[14] Al-Mansoury B.A.,Salih Y.A. .2023 .The integrated control of eggplant root rot disease caused by Fusarium solani in the greenhouse. Annals of Forest Research, 66(1) : 1441-1455.

[15] Hasan N.A.,Salih Y.A. .2022 .Efect of the bioagents Trichoderma harzianum and T. longibrachiatum on tomato fusarium wilt disease caused by Fusarium oxysporum f.sp. lycopersici and some plant growth parameters under greenhouse conditions. NeuroQuantology, 20(10) : 9816-9826.

[16] Hasan N.A.,Salih Y.A. .2023 .Evaluation of eficiency of the bioagents Trichoderma koningii and T. viride in controlling the pathogenic fungus Fusarium oxysporum f.sp. lycopersici in the pots. Misan Journal Academic Studies, 22(45) : 264-279.

[17] Rozalaska S. .2005 .Nitric oxide and hydrogen peroxide in tomato resistance: nitric oxide modulates hydrogen peroxide level in o-hydroxyethylorutin- induced resistance to Botrytis cinerea in tomato. Plant Physioogy. Biochemistry, 43 : 623-635.

[18] M.F. Abdel-Monaim M.A.,Abdel-Gaid M.A.,Armanious H.A. .2012 .Efect of chemical inducers on root rot and wilt diseases, yield and quality of tomato. International Journal of Agricultural Sciences, 7 : 211-220.

[19] J.F. Leslie B.A.,Summerell B.A.,Hoboken B.A. .2006 .. The Fusarium Laboratory Manual. Blackwell Publishing, : .

[20] M.M. .1989 .Dewan. Identity and frequency of occurrence of fungi in root of wheat and rye grass and their efect on take-all and host growth. Ph.D. Thesis, : .

[21] M.F. Ahmed .2005 .Efect of adding some biocontrol agents on non-target microorganisms in root diseases infecting soybean and broad bean plants. M.Sc. Thesis, : .

[22] R.M.S. EL-Ashmony M.R.,Abdel-Latif EL.S.,Abdou A.A.,Gala A.A. .2017 .Influence of Hydrogen peroxide (H2O2) on Trichoderma harzianum potentiality to control sunflower root/collar rot pathogen Sclerotium rolfsii. Egyptian Journal of Phytopathology, 45(2) : 103-113.

[23] Sutton L.M.,Starzyk M.J. .1972 .Procedure and analysis of a useful method in determining mycelial dry weights from agar plates. Applied Microbiology, 24(6) : 1011-1012.

[24] A.E. Dorrance S.A.,McClure S.K.,Martin S.K. .2003 .Efect of partial resistance on Phytophthora stem rot incidence and yield of soybean in Ohio. Plant Disease, 87 : 308-312.

[25] Kloepper J.W.,Tuzun S. .1995 .Induction of systemic resistance in cucumber against fusarium wilt by plant growth-promoting rhizobacteria. Phytopathology, 85 : 695-698.

[26] A.M. Lopez-Lopez R.,Allende-Molar K.C.,Correia J.M.,Tovar-Pedraza I.,Marquez-Zequera S.,Tuzun R.S.,Garcia-Estrada R.S. .2021 .First report of stem canker of tomato caused by Fusarium striatum in Mexico. Plant Disease, 105 : 497-499.

[27] M.H. Abass Q.H.,Madhi Q.H.,Matrood A.A. .2021 .Identity and prevalence of wheat damping-of fungal pathogens in diferent fields of Basrah and Maysan provinces. Bulletin of the National Research Centre, 45 : 51.

[28] Wen S.H.,Yanglan T.,Shuangxia W.,Donald M.G.,Sarah D.S.,Yucai L.,Yingying F.,Zhouping W.,Aibo W. .2017 .Mycotoxigenic potentials of Fusarium species in various culture matrices revealed by mycotoxin profiling. Toxins, 9(1) : .

[29] Kashyap A.S.,Manzar N.,Ahamad F.,Tilgam J.,Sharma P.K.,Saxena A.K. .2022 .First report of root rot disease in green gram (Vigna radiata) caused by Ectophoma multirostrata in India. Plant Disease, 106(PDIS11212400PDN) : .

[30] Ali A.A. .2018 .Role of hydrogen peroxide in management of root rot and wilt disease of thyme plan. Journal of Phytopathology and Pest Management, 5(3) : 1-13.

[31] M. Finnegan E.,Linley S.P.,Denyer G.,McDonnell C.,Simons J.Y.,Maillard J.Y. .2010 .Mode of action of hydrogen peroxide and other oxidizing agents: diferences between liquid and gas forms. Journal of Antimicrobial Chemotherapy, 65 : 2108-2115.

[32] Puyam A. .2016 .Advent of Trichoderma as a bio-control agent- A review. Journal of Appied Natural Science, 8(2) : 1100-1109.

[33] Demirici E.E.,Dane E.,Eken C. .2011 .In vitro antagonistic activity of fungi isolated from sclerotia on potato tubers against Rhizoctonia solani. Turkish Journal of Biology, 35(4) : .

[34] Gajera H.P.,Bambharolia R.P.,Patel S.V.,Khatrani T.J.,Goalkiya B.A. .2012 .Antagonism of Trichoderma spp. against Macrophomina phaseolina: Evaluation of coiling and cell wall degrading enzymatic activities. Plant Pathology and Microbiology, 3(7) : .

[35] Copes W.E. .2009 .Concentration and intervals of hydrogen dioxide applications to control Puccinia hemerocallidis on daylily. Crop Protection, 28 : 24-29.

[36] Humberto D.,Daniel C.,Luis C. .2020 .Patents on endophytic fungi for agriculture and bio and phytoremediation applications. Microorganisms, 8 : 1237.

[37] Azevedo R.A.,Gratão P.L.,Monteiro C.C.,Carvalho R.F. .2012 .What is new in the research on cadmium induced stress in plants?. Food Energy Security, 1 : 133-140.

[38] Vinale F.,Sivasithamparam K.,Ghisalberti E.,Woo S.,Nigro M.,Marra R.,Lombardi N.,Pascale A. .2014 .Trichoderma secondary metabolites active on plants and fungal pathogens. The Open Mycology Journal, 8(Suppl-1) : 127-139.

[39] Khan R.A.A.,Najeeb S.,Hussain S.,Xie B.,Li Y. .2020 .Bioactive secondary metabolites from Trichoderma spp. against phytopathogenic fungi. Microorganisms, 817(6) : .

Citations

Downloads

Views