Ultrastructure, Callus Induction and Micropropagation of Phyla nodiflora (L.) Greene. | [Pakistan Journal of Botany • 2019]

Author(s):

1. DALILAH ABU BAKAR: Advanced Biotechnology and Breeding Centre, Malaysian Palm Oil Board, 43000 Kajang, Selangor, Malaysial; Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

2. Rubashiny Veeramohan: Institute of Systems Biology (INBIOSIS), Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia

3. ABDUL BAKRUDEEN ALI AHMED: Department of Biochemistry, Centre for Research and Development (CRD), PRIST Deemed University, Vallam, India

4. NOORMA WATI HARON: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

5. ROSNA MAT-TAHA: Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

Abstract:

The present paper reports the ultrastructure and a protocol for the efficient in vitro callus induction and micropropagation on Phyla nodiflora (L.) Greene. Foliar micromorphology study of Phyla nodiflora was investigated by JEOL-7500F Field Emission Scanning Electron Microscope (FESEM). Diacytic stomata are abundant on both the adaxial and abaxial surfaces. Trichomes are simple unicellular and densely distributed. Callus induction and shoot regenerations were achieved through culturing leaf and stem explants after disinfecting with 70% ethanol 3 minutes, 0.1% HgCl2 for 3 minutes and 20% sodium hypochlorite for 2 minutes on Murashige and Skoog (MS) medium (pH5.8) supplemented with single and combinations of auxins and cytokinins, which are naphthaleneacetic acid (NAA), 2,4-dicholorophenoxyacetic acid (2,4D), indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and benzyladenine (BA), kinetin (KN). Maximum 92.5% callus response on single plant growth regulators (PGRs) is 2.5 mg/L NAA (118.6 mg/L) with light green friable callus, 90.0% response from 2.0 mg/L BA (42.7 mg/L) with green compact callus and 89.2% response from combination 3.0 mg/L NAA: 2.0 mg/L KN (165.0 mg/L) with green compact and friable callus and 2.5 mg/L IBA: 1.0 mg/L BA (139.6 mg/L) with green compact callus. Highest shoot regenerations response was from MS media without plant growth regulators (90%) while shoot regenerations from MS media supplemented with combination IAA and BA gave highest multiple shoots with 3 shoots per explants. Mass propagation of callus and plantlets protocol of Phyla nodiflora can assist for future metabolic research.

Page(s): 1695-1702

DOI: 10.30848/PJB2019-5(40)

Published: Journal: Pakistan Journal of Botany, Volume: 51, Issue: 5, Year: 2019

Keywords:

Micropropagation , in vitro , Micromorphology , Verbenaceae , Phyla nodiflora , FESEM

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views