Abstract:
Hospital dry wastes, if not disposed-off hygienically, have the potential to serve as natural reservoirs for microorganisms. In current studies, dry hospital wastes of all three layers i.e., upper, middle and lower layers of wastes dump of hospital dry wastes from the Government Central Hospital, Saidu Sharif and Saidu Teaching Hospital, district Swat were examined for isolation of pathogenic bacteria. The biochemical tests revealed that Klebsiella pneumonia, Salmonella enterica, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus cerus, Exiguobacterium, and Pseudo Ochrobactrum Asaccharolyticus were the predominant bacteria isolates from all three layers of wastes dump. The highest number seven isolates of Klebsiella pneumonia and Salmonella enterica, Six isolates of Bacillus cerus, E. coli and Pseudomonas aeruginosa were isolated. That were followed by Exiguobacterium, and Pseudo chrobactrum asaccharolyticus with 3 isolates. While the lowest number was of Staphylococcus aureus with 2 isolates. Klebsiella pneumonia and Salmonella enterica were isolated from all three layers. Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus cerus, Exiguobacterium from the middle and bottom layers. Whereas, Exiguobacterium and Pseudochrobactrum asaccharolyticum were isolated from the single bottom layer of wastes dump. For the antibiogram studies, antibiotics were selected from CLSI panel for specific isolate. The results revealed that out of ten Cefipime, Meropenem, Amoxycline were completely resistant and Fosfomycin, Nulidixic acid showed the intermediate against K. pneumonia. Similar, among 12 tested antibiotics against Salmonella enterica, the Amoxicilin, Cefixime, Ceftriaxone, Fosfomycin and linezolid are completely resistant. Against S. aures 5 out of 18 i.e., Septran, Chloramphenicol, Linazolid, Novobiocin, Ciprofloxacin were effective. Eshiresha coli showed resistance against 9 out of 10 and only Fosfomycin was effective. All biochemical identified isolates were further confirmed though the molecular sequencing of the 16sRNA using universal primers. The obtained sequences were BLAST in NCBI for phylogenic analysis. The data showed that our query sequences from the isolates were closer to the identified base strains, reported from different regions of the world including Pakistan. The two strains i.e., Exiguobacterium and Pseudochrobactrum asaccharolyticus were first time reported from Pakistan.
Page(s):
36-36
DOI:
DOI not available
Published:
Journal: Abstract Book on International Conference on Life Sciences (ICLS-23) 11-12 May 22-23, Volume: 0, Issue: 0, Year: 2023
Keywords:
Wastes
,
bacterial diversity
,
Hospital
,
Dry
,
Exiguobacterium and Pseudo Ochrobactrum Asaccharolyticus