Agrobacterium-Mediated Genetic Transformation System of Amorpha fruticosa using Callus from the Cotyledonary Node | [International Journal of Agriculture and Biology • 2022]

Author(s):

1. Yiteng Zhang: Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University,Harbin,China

2. Jiali Liu: Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University,Harbin,China

3. Ailing Zhong: Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University,Harbin,China

4. Ziang Liu: Forestry Institute, Northeast Forestry University,Harbin,China

5. Kai Wang: Forestry Institute, Northeast Forestry University,Harbin,China

6. Zhenyu Wang: Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences,Harbin,China

7. Minghui Li: Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University,Harbin,China

8. XiuFeng Li: Northeast Institute of Geography and Agroecology, Key Laboratory of Soybean Molecular Design Breeding, Chinese Academy of Sciences,Harbin,China

9. Qingjie Guan: Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University,Harbin,China

Abstract:

We used Agrobacterium-mediated infection of callus induced from the cotyledonary nodes of Amorpha fruticosa L. to study the ß-glucuronidase gene (GUS)-integrated genetic transformation system. Transformed calluses were selected under 40 mg·L-1 kanamycin, differentiated into resistant adventitious buds, and developed into transformants. A single copy of gus was integrated in the genome of most T0 generation plants. Gus chemical staining analysis showed blue color in resistant calluses, adventitious buds, and the roots and leaves of transformed plants. This indicated gus overexpression driven by the 35S promotor and resultant ß-glucuronidase activity. The genetic transformation system in this study could be used to study other functional genes of A. fruticosa and facilitate transgenic breeding for strain improvement.

Page(s): 203-208

DOI: 10.17957/IJAB/15.1917

Published: Journal: International Journal of Agriculture and Biology, Volume: 27, Issue: 3, Year: 2022

Keywords:

Genetic transformation , Agrobacterium , Callus , Cotyledonary node , Amorpha fruticosa L

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