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A simple method for the simultaneous extraction of cholesterol and phosphatides from reference bovine to spinal phosphatidylcholine cord with particular and phosphatidylethanolamine.
Author(s):
1. Qamar Khalid: Pakistan Council of Scientific & Industrial Research, PCSIR Laboratories Complex, Karachi, Pakistan
2. Liaquat Sultana: Pakistan Council of Scientific & Industrial Research, PCSIR Laboratories Complex, Karachi, Pakistan
3. Nayeemuddin: Pakistan Council of Scientific & Industrial Research, PCSIR Laboratories Complex, Karachi, Pakistan
Abstract:
A large-scale process is described for the simultaneous extraction of cholesterol, phosphatidyl choline (PC) and phosphatidyl ethanolamine (PE) from bovine spinal cord. The process is essentially of batch type and consists of four stages, each requiring a different solvent. Total lipids are first extracted from the minced and dried spinal cord with n-hexane. The concentrated extract is then stirred with n-hexane: acetone mixture (10.90 v/v) to separate cholesterol from the phosphatides which are filtered in the Sparkler's filter under inert atmosphere. Cholesterol is recovered from the acetone extract by distillation and crystallisation from methanol. Phosphatides are extracted with ethanol to selectively remove PC and PE by recycling ethanol through the Sparkler's filter. The ethanol extract is chilled overnight and the precipitated PE filtered off. PC is recovered by distilling the solvent under vacuum. The recovery of cholesterol PC and PE on dry weight basis is 12, 10 and 1% respectively. The process is comparatively simple and economically feasible for a large-scale production of the title products.
Page(s): 4-8
DOI: DOI not available
Published: Journal: Pakistan Journal of Scientific and Industrial Research, Volume: 29, Issue: 1, Year: 1986
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