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Marker-assisted screening of wheat (Triticum aestivum L.) Genotypes for stripe rust resistance
Author(s):
1. Basit Ali: Institute of Biotechnology & Genetic Engineering , The University of Agriculture,Peshawar, 25130, Khyber Pakhtunkhwa,Pakistan
2. Hilal Ahmad: Institute of Biotechnology & Genetic Engineering , The University of Agriculture,Peshawar, 25130, Khyber Pakhtunkhwa,Pakistan
3. Quahir Sohail: Institute of Biotechnology & Genetic Engineering , The University of Agriculture,Peshawar, 25130, Khyber Pakhtunkhwa,Pakistan
Abstract:
Yellow rust, caused by Puccinia striiformis, is a major disease contributing to significant wheat yield losses around the world including Pakistan. Genetic resistance is the key- explain. This study assessed agronomic and molecular traits associated with yellow rust resistance in 38 wheat genotypes using SSR markers. Five key agronomic parameters; plant height, spike length, grain per spike, grain weight per spike and 1000-grain weight were evaluated. The genotypes Wadaan-17, NIA-Zarkhaiz and PR-155 showed maximum plant heights. PR-155, Pirsabak-19 and Wadaan-17 had the longest spikes. Taskeen-22, Paseena-17 and Abdul-Bari 24 produced the highest number of grains spike-1. Taskeen-22, Tarnab Rehber, and Wafaq-23 exhibited the highest grain weightspike-1. For 1000-grain weight, the highest values were recorded for NIFALalma-13, Tarnab Rehber, and Pirsabak-21. Yellow rust resistance was assessed using the Average Co-efficient of Infection (ACI) and Relative Resistance Index(RRI), with genotypes showing a broad range of disease severity. Resistant genotypes exhibited ACI values between 0-9.67%, while susceptible genotypes from 21%- 36%. The RRI values ranged from 0-9, with resistant genotypes scoring between 8.5-9.0, while susceptible genotypes scored between 0-3.8.Molecular analysis using six SSR primers linked to Yr genes identified four markers (Yr5, Yr15, YrSP, Lr46) with distinct bands, confirming the presence of yellow rust resistance genes. The Yr5 primer exhibited the highest molecular frequency (86.84%), while Yr15 had a lower frequency of 44.7%. The study highlights the importance of combining yellow rust resistance and high yield traits for developing wheat varieties to address food security challenges
Page(s): 155-155
DOI: DOI not available
Published: Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
agronomic traits , molecular markers , wheat , Yellow rust , ACI , Triticum aestivum L , RRI
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