Triple test cross analysis revealed the absence of epistasis in the inheritance for clcud in cotton | [Pakistan Journal of Botany • 2025]

Author(s):

1. FAKHAR-UZ-ZAMAN KHAN: Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences & Technology (FAST), Bahauddin Zakariya University,Multan,Pakistan

2. ABDUL QAYYUM: Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences & Technology (FAST), Bahauddin Zakariya University,Multan,Pakistan

3. WAQAS MALIK: Department of Plant Breeding and Genetics, Faculty of Agricultural Sciences & Technology (FAST), Bahauddin Zakariya University,Multan,Pakistan

4. MAMOONA HANIF: Cotton Research Institute,Multan,Pakistan

Abstract:

The modified triple test cross technique was employed to analyze the presence of dominance, additive and epistasis components contributing to the inheritance of traits and cotton leaf curl disease (CLCuD) in cotton. The study unveiled the significance of epistasis as a critical contributor in the inheritance of biochemical traits under CLCuD impact. Among epistasis the additive x additive (i) and the additive × dominance + dominance × dominance (j + l), played pivotal roles in shaping trait expression. Gene action, predominantly additive and dominance types, significantly influenced the manifestation of CLCuD, chlorophyll-b, and carotenoids. Interestingly, no indication of directional dominance was observed for these traits. Regarding chlorophyll-a, total chlorophyll, Superoxide Dismutase, Peroxidase, Catalase, and total soluble protein, a strategic approach involving recurrent selection or biparental mating is recommended, particularly starting from the F2 generation to improve many traits. However, to harness the full potential of epistatic effects and foster the development of desired cultivars in cotton, deferring the selection of desired plants until the F5 or F6 generations is advisable. This deliberate delay enables the maximum fixation of epistatic effects, crucial for cultivating desired cotton cultivars resilient to CLCuD.

Page(s): 1753-1759

DOI: 10.30848/PJB2025-5(35)

Published: Journal: Pakistan Journal of Botany, Volume: 57, Issue: 5, Year: 2025

Keywords:

Biochemical Traits , Epistasis , Triple test cross analysis , Cotton breeding , Cotton leaf curl disease CLCuD

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