The use of a Box–Behnken design to optimize the extraction technology of Phyllanthus emblica L. polyphenols and simultaneous compositional analysis using HPLC with fluorescence detection. | [Journal of Chemical Society of Pakistan • 2015]

Author(s):

1. Hongyang Pan: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, P. R. China

2. Tougen Liao: Hongyunhonghe research institute of fragrance and flavor industry, Kunming 650231, P. R. China, Technology Center, China Tobacco Yunnan Industrial Co, Ltd, Kunming 650231, P. R. China

3. Baokun Zhu: Hongyunhonghe research institute of fragrance and flavor industry, Kunming 650231, P. R. China, Technology Center, China Tobacco Yunnan Industrial Co, Ltd, Kunming 650231, P. R. China

4. Yupeng Gong: Hongyunhonghe research institute of fragrance and flavor industry, Kunming 650231, P. R. China, Technology Center, China Tobacco Yunnan Industrial Co, Ltd, Kunming 650231, P. R. China

5. Liang He: Hongyunhonghe research institute of fragrance and flavor industry, Kunming 650231, P. R. China, Technology Center, China Tobacco Yunnan Industrial Co, Ltd, Kunming 650231, P. R. China

6. Mingfeng Wang: Hongyunhonghe research institute of fragrance and flavor industry, Kunming 650231, P. R. China, Technology Center, China Tobacco Yunnan Industrial Co, Ltd, Kunming 650231, P. R. China

Abstract:

Extractions of polyphenols from Phyllanthus emblica L. were performed by supercritical CO2 fluid extraction (SFE). A three-level Box–Behnken factorial design using response surface methodology was applied to optimize the main extraction conditions; including the ratio of the solvent to raw material; pressure; extraction temperature and extraction time on the PEP yields. The results showed that the optimal conditions of the CO2 flow rate was 35.9 l/h; pressure was 90.1 MPa; the extraction temperature was 55°C and the extraction time was 1.1 h. Under optimum conditions; PEP yields of 84.19±0.51 mg GAE/100 g were obtained; which closely matches the predicted value of the yield. Simultaneous determination of nine polyphenol compounds in polyphenols using HPLC with fluorescence detection has been successfully achieved. HPLC analysis indicated that the major polyphenols in the SFE extracts consisted of chlorogenic acid; caffeic acid; syrigin; procyanidin B2; (-)-epicatechin; cinnamic acid; coumaric acid; phlorizin and quercetin; of which procyanidin B2 had the highest content of 231.77 mg/kg.

Page(s): 1153-1161

DOI: DOI not available

Published: Journal: Journal of Chemical Society of Pakistan, Volume: 37, Issue: 6, Year: 2015

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