the proliferation and migration-enhancing effects of vitronectin in SMMC 7721 cells: A pilot study. | [Journal of Basic and Applied Sciences • 2014]

Author(s):

1. Wei Zhu: Departmant of Toxicology, Guangzhou Center for Disease Control and Prevention, Guangzhou, China

2. Konghe Hu: Department of orthopaedics, The Affiliated Yue Bei People`s Hospital of Shantou University Medical College, Shaoguan, China

3. Wenxue Li: Departmant of Toxicology, Guangzhou Center for Disease Control and Prevention, Guangzhou, China

4. Guangyu Yang: Departmant of Toxicology, Guangzhou Center for Disease Control and Prevention, Guangzhou, China

5. Jianling Chen: Guangdong Prevention and Treatment Center for occupational Disease, Guangzhou, China

6. Chuanxi Fu: Departmant of Toxicology, Guangzhou Center for Disease Control and Prevention, Guangzhou, China

7. Qiansheng Hu: Faculty of Toxicology, School of Public Health, Sun Yet-sen University, Guangzhou, China

Abstract:

To understand the effects of Vitronectin on cell proliferation and migration in the cell line of hepatocellular carcinoma, SMMC 7721, the effects of Vitronectin on cell proliferation rate or on the prevention of the cells from the apoptotic stimuli were appraised with WST-1 assay; and the morphology of β-Tubulin was observed with con-focal microscope. The effect on migration was detected with transwell chamber. The results show that Vitronectin helps the cells adhere to Petri dish as well as the sustaining of the morphology of β-Tubulin. Vitronectin could enhance the proliferation rate of SMMC 7721 with the concentration-effect mode, and could protect the cells from the stimuli of apoptosis inducer. As to cell migration, the results show that Vitronectin enhance cell migration across the membrane of transwell chamber. According the results, the conclusion could be made that Vitronectin might play important roles in the following biological effects, such as sustaining the morphology of the tumor cells, enhancing the proliferation as well as the migration.

Page(s): 205-211

DOI: DOI not available

Published: Journal: Journal of Basic and Applied Sciences, Volume: 10, Issue: 0, Year: 2014

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