Author(s):
1. Hafiza Sehrish Kiani:
Department of Microbiology, Quaid-i-Azam University,Islamabad,Pakistan
2. Muhammad Ishtiaq Ali:
Department of Microbiology, Quaid-i-Azam University,Islamabad,,Pakistan
3. Hassan Waseem:
Muslim Youth University,Islamabad,Pakistan
Abstract:
Hospital wastewater are the hotspot for the antibiotic resistance dissemination into the environment as it leads to ARB proliferation and horizontal genes transfer among microorganisms. The current study focuses on the antibiotic resistant gene isolation and identification from the environmental sample of hospital wastewater and their knockout by two stage anaerobic digestion process. Strategic optimization via controlling parameter that is the effect of different temperature (22°C, 37°C, 46°C, 55°C) on fate of ARGs. The current study was conducted to isolate and identify the antibiotic resistant genes from hospitals wastewater and assessing the anaerobic digestion process impact on antibiotic resistant genes by performing double stage anaerobic digestion process. Additionally, the study was done to collect samples from 3 different hospitals for identification of biological and technical replicates and to obtain the pure/isolated colonies via biochemical identification. To evaluate phenotypical confirmation by performing susceptibility testing before and after wastewater treatment. To employ PCR testing for genotypic confirmation before and after wastewater treatment. To perform anaerobic digestion process in double stage anaerobic digesters. Wastewater sample collection according to SMPs from three different hospitals. After serial dilution, total bacterial count was performed to determine total culture able bacterial number and diversity among samples. Biochemical identification was done. Isolation of ARBs occurred via antibiotic sensitivity testing ARGs identification via PCR. Wastewater was treated in double stage anaerobic digesters for about 90 days. After WWT, susceptibility testing for phenotypical confirmation; PCR for genotypic confirmation. At the end, sequencing was performed for checking mutation. Significant ARGs removal has been observed from hospital wastewater via optimized conditions in anaerobic digestion process. Higher thermophilic temperature-55°C have more promising effects on fate of ARGs removal instead of mesophilic temperature. High density and diversity of microorganism sustained by nutrient rich environments of HWW creates favorable conditions for proliferation 6f ARBs and ARGs transfer. Significant removal of ARGs proves that anaerobic bioreactors are far better than conventional WWTP.
Page(s):
0-0
DOI:
DOI not available
Published:
Journal: First International Conference on Revamped Scientific Outlook of 21st Century (Abstract Book), Volume: 0, Issue: 0, Year: 2022
Keywords:
Wastewater treatment
,
SMPs
,
WWTPs
,
ARG
,
ARB proliferation
,
HWW
References:
References are not available for this document.
Citations
Citations are not available for this document.