Author(s):
1. Imran Rauf:
Nuclear Institute of Agriculture (NIA) Tandojam, Pakistan
2. Raza Muhammad Memon:
Nuclear Institute of Agriculture (NIA) Tandojam, Pakistan
3. Noroza Umar:
National Institute of Biotechnology and Genetic Engineering (NIBGE) Faisalabad, Pakistan
4. Rubab Zahra Naqvi:
National Institute of Biotechnology and Genetic Engineering (NIBGE) Faisalabad, Pakistan
5. Imran Amin:
National Institute of Biotechnology and Genetic Engineering (NIBGE) , Pakistan
6. Shahid Mansoor:
National Institute of Biotechnology and Genetic Engineering (NIBGE) Faisalabad, Pakistan
Abstract:
In herbivore insects, RNAi can be achieved by feeding target insects on plants produce double standard RNA (dsRNAs) either by stable or transient transformation. Production of dsRNAs through stable transformation is a slow and laborious process, so, here we demonstrated a quick and efficient transient method. We used Potato Virus X (PVX) as a viral vector to produce dsRNAs transiently in the host plant Nicotiana tabacum which cause quick gene knockdown of target gene (chitin synthase) in herbivore insect, mealybug (Phenacoccus solenopsis). Reverse-transcriptasepolymerase chain reaction RT-PCR validate the expression of transgene in recombinant-PVX-inoculated plants, whereas, population of mealybug was significantly reduced (30%) just after 48hrs of feeding on recombinant PVX infected plants and reached upto 78% in 7-9days. The quick knockdown of target gene and mortality of the insects feeding on recombinant PVX infected plant validate that the recombinant PVX is an effective tool for evaluating candidate RNAi effectors in plants.
Page(s):
673-679
DOI:
DOI not available
Published:
Journal: International Journal of Biology and Biotechnology, Volume: 18, Issue: 4, Year: 2021
Keywords:
RNAi
,
control
,
VIGS
,
Mealybug
,
PVX virus
,
transient expression
References:
References are not available for this document.
Citations
Citations are not available for this document.