An extract of Perilla stem inhibits SRC homology phosphatase-1 (SHP)-1 and influences insulin signaling. | [Pakistan Journal of Pharmaceutical Sciences • 2015]

Author(s):

1. Liu Peng: Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China;The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China

2. Zhang Lei: Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China;The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China

3. Xie Xiao-na: The First Hospital of Jilin University, Changchun, PR China

4. Wang Deli: The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China

5. Sun Jing: Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China;The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China

6. Wang Yong-sen: Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China;The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China

7. Wang Zhi: Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China

8. Xing Shu: Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China;The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China

9. Ma Jun-feng: Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China;The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China

10. Li Wan-nan: Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China;The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China

11. Fu Xue-Qi: Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, Jilin University, Changchun, PR China;The State Engineering Laboratory of AIDS Vaccine, Jilin University, Changchun, RP China

Abstract:

Protein tyrosine phosphatases (PTPs) are enzymes that catalyze protein tyrosine dephosphorylation of which Src homology phosphatase-1 (SHP-1) is one of the best-validated, a widely distributed intracellular tyrosine phosphatase that contains two SH2 domains. Down regulation of SHP-1 tyrosine phosphatases was significantly increased sensitivity to insulin in insulin signaling pathway. Through in vitro enzymatic reaction kinetics experiment, we found that the extract of Perilla stem was a potential inhibitor to ?SHP-1, the catalytic domain of SHP-1 protein tyrosine phosphatase, and its IC50 was 4ug/ml, and was more sensitive towards SHP-1than other PTPs, which indicated that SHP-1 might be a target of the extract of Perilla stem. It can strengthened the level of tyrosine phosphorylation of insulin receptor (IR) and extracellular signal-regulated protein kinase (ERK) in HepG2 cells, and then activated the insulin signaling pathway through inhibiting the protein phosphorylation of SHP-1. These results demonstrated that the extract of Perilla stem could play an important role for diabetes treatment through inhibiting the level of SHP-1 in insulin signaling pathway.

Page(s): 421-424

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 28, Issue: 2, Year: 2015

Keywords:

Keywords are not available for this article.

References:

[1] Peng Liu,Wang Liu .2015 .An extract of Perilla stem inhibits Src homology phosphatase-1(SHP)-1 and influences insulin signaling. Pak. J. Pharm. Sci., 28(2) : 421-424.

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