The LC-MS/MS profiling of AHLs produced in Sinorhizobium meliloti nodulating Alysicarpus bupleurifolius. | [Pakistan Journal of Botany • 2013]

Author(s):

1. Samiullah Khan: Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan

2. Abdul Samad Mumtaz: Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan

3. Ghulam Mustafa: Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan

4. M. Naveed: Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan

5. Zabta Khan Shinwari: Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan

6. Allan Downie: John Innes Centre, Norwich Research Park, Norwich, United Kingdom

Abstract:

We isolated Sinorhizobium meliloti (PCC21) from root nodules of wild legume species Alysicarpus bupleurifolius and characterized the AHL diversity produced in symbiosis. The TLC and HPLC-MS/MS profiles revealed production of a diverse array of AHLs that ranged from short chain C4-HSL to long chain C12-HSL. It was observed that isolates in young nodules produced AHL abundance. The study reports a novel symbiotic association between S. meliloti and Alysicarpus bupleurifolius and points to the possible presence of more than one synthase systems, as previously described in Rm1021 and AK63. The isolation of a strain producing wide ranging AHL molecules provides an opportunity to investigate the complex genetics of AHL production in S. melitoti.

Page(s): 2037-2041

DOI: DOI not available

Published: Journal: Pakistan Journal of Botany, Volume: 45, Issue: 6, Year: 2013

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