A new species of Inosperma, and first record of I. afromeliolens from West Africa | [Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 • 2023]

Author(s):

1. Hyppolite L. Aïgnon: Research Unit Tropical Mycology and Plants-Soil Fungi Interactions, Faculty of Agronomy, University of Parakou, 03 BP 125, Parakou, Benin

2. Yu-Guang Fan: Key Laboratory of Tropical Translational Medicine of Ministry of Education Tropical Environment and Health Laboratory, School of Pharmacy, Hainan Medical University, Haikou 571199, China

3. André De Kesel: Meise Botanic Garden, Nieuwelaan 38, 1860 Meise, Belgium

4. Martin Ryberg: Systematic Biology program, Department of Organismal Biology, Uppsala University, Norbyvägen 18D, 752 36 Uppsala, Sweden.

5. Nourou S. Yorou: Research Unit Tropical Mycology and Plants-Soil Fungi Interactions, Faculty of Agronomy, University of Parakou, 03 BP 125, Parakou, Benin

Abstract:

Inosperma (Kühner) Matheny & Esteve-Rav., is monophyletic and four distinct clades have been recognized within the genus: two distinct Tropical Old World clades (1 and 2), the Maculata clade, and Inosperma sect. Inosperma. The specimens analysed in this study were collected between 1997 and 2022 in Benin (West Africa) in woodlands dominated by Isoberliniadoka Craib & Stapf, and/or I. tomentosa (Harms) Craib & Stap f, or gallery forest dominated by Uapacaguineensis Müll. Arg and/or Berlinia grandiflora (Vahl) Hutch. & Dalziel, in Okpara Forest (9.270131°N, 2.715440°E), N'dali Forest Reserve (9.758857°N, 2.696819°E), Koussoucoingou gallery forest (10.173066°N, 1.196233°E), Forest reserve of Gbadji (7.952167°N, 1.967867°E) and Forest reserve of Wari Maro (9.164733°N, 2.159917°E). The fresh basidiomata were dried using an electric dryer type Stöckli Dörrex for 24 hours at 60°C. Genomic DNA was extracted from dried specimens collected using the NuClean Plant Genomic DNA kit (ComWin Biotech, Beijing) and the ITS, LSU, RPB2 and TEF1 were amplified. The muscarine content was estimated in the UPLCMS/MS by using standard muscarine (Sigma-Aldrich, Chemical purity = 98%), and was calculated with an external standard method based on their respective calibration curves. The results show Inosperma macrocarpa as new to the science and the first record of I. afromelliolens from West Africa. The phylogenetic placement of I. macrocarpa was inferred, based on molecular evidence from the sequences of ITS, LSU, RPB2 and TEF1 and indicate that I.macrocarpa is nested an Old World Tropical clade 1. Complete description and illustrations, including photographs and line drawings, are presented for the new species. Morphological and molecular analysis based on collections from Benin also confirms the presence of I. afromelliolens in West Africa. A toxicity analysis shows that neither species contain muscarine which lend further support to the hypothesis that the ability to produce muscarine is a derived trait in Inosperma.

Page(s): 19-19

DOI: DOI not available

Published: Journal: Abstract Book on Second International Conference on Recent Approaches in Plant Sciences (RAPS-23) 4-5 May 2023 , Volume: 0, Issue: 0, Year: 2023

Keywords:

Taxonomy , New species , Phylogeny , Taxonomy , Molecular systematics , Basidiomycota , muscarine

References:

References are not available for this document.

Citations

Citations are not available for this document.

Citations

Downloads

Views