Author(s):
1. Aiman Khan:
Department of Biotechnology, Abdul Wali Khan University Mardan,Pakistan
2. M. Ihtisham:
Department of Biotechnology, Abdul Wali Khan University Mardan,Pakistan
3. Murad Ali:
Department of Biotechnology, Abdul Wali Khan University Mardan,Pakistan
4. Amjad Iqbal:
Department of Food Science & Technology, Wali Khan University Mardan,Pakistan
5. Ayaz Ahmad:
Department of Biotechnology, Abdul Wali Khan University Mardan,Pakistan
Abstract:
Pathogenic diseases share reasonable burden on human health. Emergence of resistance to antimicrobial drugs is one of the major concerns because of their indiscriminate utilization. Besides plants have supplied mankind with all the basic nutrients requirements, they have also served as a good source of bioactive compounds that serve as a medicine. Lepidium sativum, commonly known as garden cress is a medicinally important plant that comprises of bioactive compounds to promote human health. Polysaccharide is one of such bioactive ingredients that is abundantly present in garden cress. The aim of the study was to isolate the water-soluble polysaccharides from cress seed mucilage through gel permeation chromatography and to evaluate their antimicrobial potential. Mucilage from the cress seeds was obtained by soaking the seeds in autoclaved distilled water for overnight. The polysaccharides were then collected in the form of precipitates after treating the mucilage with 75% ethanol. The precipitated fraction was re-dissolved in double distilled water (ddH2O) and fractionated through Bio-Gel-P-10 having pore size of 90 µm-180 µm. A total of 60 fractions were obtained and each fraction was screened for its biochemical composition. The results showed the presence of total carbohydrates, saturated and unsaturated uronic acid, pentoses and hexoses. The antibacterial potential of the selected fractions was determined by using agar well diffusion, microbial biofilm and minimal inhibitory concentration assay against five different human pathogenic strains. The results showed that fraction Cr20 at 100 µg/mL was able to induce 6.78 mm and 6.7mm zone of inhibition against S. aureus and S. typhi. Inhibition of the microbial biofilm formation was investigated for all the selected 60 fractions, where Cr22 fraction exhibited significant (P=0.05) activitywithIC50 value of 14.67 µg/mL against Klebsiella. The results of minimal inhibitory concentration assays revealed that the fraction Cr20 inhibited the growth of Shigella, Klebsiella and Salmonella at a concentration of 57.59 µg/mL. The findings concluded that water soluble polysaccharides from cress seed mucilage can work best against the human pathogenic microorganisms. Further elucidation and tests of the potent fractions may lead to the development of novel antimicrobial drugs.
Page(s):
103-103
DOI:
DOI not available
Published:
Journal: International Food & Nutrition Conference" August 18th-20th, 2022, University of Swat, Swat (Book of Abstracts), Volume: 1, Issue: 1, Year: 2022
Keywords:
antimicrobial
,
mucilage
,
Cress seed
,
Polysaccharides
References:
References are not available for this document.
Citations
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