Author(s):
1. Umer Sadique:
College of Veterinary Sciences, The University of Agriculture, Peshawar, Pakistan
Abstract:
Whilst food-producing-animals could be a pool of resistance-conferring elements in the existing animal production system in Pakistan, the issue has not yet judiciously been highlighted. This study was conducted to determine incidence of extended spectrum ß lactamase (ESBL) - producing Escherichia coli in table eggs and human with history of close association with table eggs. For this purpose, a total of 200 table eggs and 50 stool samples from human were analyzed. Results showed that out of 80 E. coli isolates recovered from eggs, 20 (25%) were found to be ESBL-producers, while, of the 17 human isolates, 4 (23.5%) were ESBL producers. PCR revealed that blaCTXM (blaCTXM-1=15 and blaCTXM-9=4) was carried by all 22 (91.6%) ESBL-producers with additional blaSHV2 (n=12) and blaNDM-1 (n=3), but no blaTEM was identified. A predominant combination of blaCTXM + blaSHV2 (n=12) followed by blaCTXM + blaNDM-1 (n=3) was determined. All these isolates (phylogroup D=14/24, A= 6/24 and B2=4/24) were found to be multidrug resistant displaying resistance against at least three different classes of antibiotics. Class 1 integron was carried by (21/24) followed by additional class 2 integron (15/24). A total of 8 isolates were harboring insertion sequence common region 1 (ISCR1), which was found to be linked with blaCTXM in 50% (4/8) isolates. Results of the current study indicate contamination of eggs with ESBL-producing E. coli suggesting to improve hygienic process for end consumer during- egg production.
Page(s):
151-151
DOI:
DOI not available
Published:
Journal: International Food & Nutrition Conference" August 18th-20th, 2022, University of Swat, Swat (Book of Abstracts), Volume: 1, Issue: 1, Year: 2022
Keywords:
PCR
,
multidrug resistant
,
Escherichia coli
,
ß lactamase
,
table eggs
References:
References are not available for this document.
Citations
Citations are not available for this document.