An exploratory study of enantioselective behavior of Sol-Gel encapsulated human serum albumin using frontal analysis | [Pakistan Journal of Pharmaceutical Sciences • 2019]

Author(s):

1. Marcela Hurtado y de la Pena: Departamento de Química Analítica,Facultad de Química, UNAM, Ciudad Universitaria, México D.F.,Mexico; Departamento Sistemas Biológicos, UAM-Xochimilco. Calzada del Hueso,Col. Villa Quietud, México D.F.,México

2. Rosario Covarrubias Herrera: Departamento de Química Analítica,Facultad de Química, UNAM, Ciudad Universitaria, México D.F.,Mexico

3. Alma Luisa Revilla Vazquez: División de Ciencias Químico Biológicas, Facultad de Estudios Superiores Cuautitlán,UNAM, Campo1, Av. 1° de Mayo s/n Col. Santa María las Torres Cuautitlán Izcalli Edo de Mexico,Mexico

Abstract:

Adsorption behavior of pure enantiomers and racemic mixtures of nonsteroidal anti-inflammatory drugs (ibuprofen and naproxen) on human serum albumin (HSA) was evaluated. The HSA was immobilized by Sol-Gel technique and this biomaterial was used in a chromatographic system where frontal analysis experiments were performed at pH 7.4 and temperatures of 25°C and 37°C. The association constants for enantiomers of the drugs were determined by linear adjustment for data corrected just for dead volume. In uncorrected data for non-specific retention, an inverse ratio between the number of sites and the value of the association constant was found. The participation of non-specific retention was estimated by non-linear regression and the values of association constants (Kass), which were determined considering this information, are comparable to some values reported by other methods at 37°C: 1.4 x105 and 5.7 x104 for Ibuprofen (IBU) R and S, respectively, and 2.3 x105 and 1.8x105 for naproxen (NX) R and S, respectively.

Page(s): 2717-2724

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 32, Issue: 6, Year: 2019

Keywords:

Solgel , enantioselectivity , Chiral stationary phase , Association constant , frontal analysis

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