Abstract:
Endophytes are microbes that colonize living, internal tissues of plants without causing any negative effects. In recent years, entophytic fungi have been demonstrated to be excellent exo polysaccharide (EPS) producers. EPS are high molecular-weight carbohydrates with a wide range of biological activities. This study was, therefore, designed to extract and purify EPS-producing endo phytic fungi from wheat (Triticum aestivum) and Parthenium (Partheniumhysterophorus) and explore their biological applications using different bioassays. The purified EPS were labelled as EPSW1, EPSW2, EPSW3, EPSP1,EPSP2, and EPSP3. The purification of EPS involved inoculating isolated endo phytic fungi into a broth medium at 25°C for 32 hours on a shaking incubator and EPS precipitation using 95% ice-chilled ethanol. The purified EPS were processed for quantification of total carbohydrate content and bioactivity through a-amylase, a-glucosidase, and HBRCs membrane stabilization assay. The results indicated that EPSW1 inhibited a-amylase, showing an effectiveIC50 value of 265.75 µg/mL, while EPSP2 inhibited a-glycosidase with an IC50value of 441.87 µg/mL. In terms of in vitro anti-inflammatory activity, EPSP1significantly stabilized HRBCs membrane, with an EC50 value of 8.75 µg/mL ,followed by EPSW1 (155.17 µg/mL). Furthermore, the study assessed in vivo anti-inflammatory activity through a carrageenan-induced paw edema model in mice. Simultaneously, the in vivo anti diabetic activity was investigated in Balb/C mice by inducing diabetes via a 1% alloxan injection. Three concentrations (400 mg/kg, 200 mg/kg, 100 mg/kg) were tested for each activity. At 400 mg/kg, EPSP1 showed the highest anti-inflammatory impact(91.37%) while EPSW2 at the same concentration displayed a significant74.85% reduction in blood glucose levels in all oxan-induced diabetic mice. In conclusion, the study emphasizes the potent bioactivity of EPS from wheat and Parthenium, showcasing therapeutic benefits, including anti-diabetic and ant iinflammatory properties.
Page(s):
130-130
DOI:
DOI not available
Published:
Journal: 1st International Conference on "Recent Advances in Green Biotechnology and Climate Resilience", September 15-16, 2025, Volume: 1, Issue: 1, Year: 2025
Keywords:
Antiinflammatory
,
Wheat
,
Antidiabetic
,
exopolysaccharides
,
Endophyte
,
Parthenium