Abstract:
TB is infectious air borne disease caused due to a pathogenic bacteria Mycobacterium Tuberculosis (M.tb). Transferred mainly through coughing, close personal contact etc. 24th march is celebrated as world TB Day because on same day in 1882 Robert Koch described the cause of disease M.tb. It is included in list of top 10 disease that causes major percentage of deaths every year. It causes more than million deaths every year alt global level. Different tests are used to diagnose disease at different stages and in different age groups like at initial stages of disease chest radiography is used, skin testing etc. many groups of anti-TB drugs are used to treat disease. It is more common in people having compromised immune system like patients having HIV. Coinfection of HIV with disease makes it more difficult to treat disease. Control and prevention of disease is mainly hindered by drug resistance in causative agent and latent form. Since more than a century BCG is only drug which is licensed to use against disease. It is only effective in children not in adults. Different new strategies are used to produce vaccine against TB which are more effective. DNA vaccines are more focused in research areas to produce vaccine now a days. Recombinant DNA technology is mainly used in production of DNA vaccines. DNA vaccines leaves long lasting affects in body, are safer, stable and can easily be prepared in research institutes. This study is designed to prepare DNA vaccine using a specific gene of M.tb i.e., Rv0577. Gene Rv0577 was amplified using standard PCR method with manually designed primers. Gene and vector pVAX1 were digested using specific enzymes (HindIII and XbaI) and was cloned to vector directly. For transformation chemically prepared competent cells from DH5a strain of E.coli. clone was extracted and confirmed by standard confirmation methods i.e., digestion, PCR, and sequencing of clone. clone was further used with different combinations in mice trial. Serum was separated from mice blood extracted during mice trial and used in different assays. Different immune responses and antibody production was studied.
Page(s):
79-79
DOI:
DOI not available
Published:
Journal: Abstract Book on Global Science Technology and Management Conference, Volume: 0, Issue: 0, Year: 2023