Transcriptome and Physiological Analysis of Germination in Gibberellic Acid-primed Tobacco Seeds. | [International Journal of Agriculture and Biology • 2018]

Author(s):

1. Jie Zhang: Molecular Genetics Key Laboratory of China Tobacco, Guizhou Academy of Tobacco Science, Guiyang, 550081, China: Key Laboratory of Tobacco Quality in Guizhou Province, Guizhou University, Guiyang, 550025, China

2. Yiling Liu: College of Agriculture, Guizhou University, Guiyang, 550025, China: Key Laboratory of Tobacco Quality in Guizhou Province, Guizhou University, Guiyang, 550025, China

3. Xuemei Du: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China

4. Jiehong Zhao: Molecular Genetics Key Laboratory of China Tobacco, Guizhou Academy of Tobacco Science, Guiyang, 550081, China

5. Jianhua Wang: College of Agriculture and Biotechnology, China Agricultural University, Beijing, China

6. Zhenhua Li: College of Agriculture, Guizhou University, Guiyang, 550025, China: Key Laboratory of Tobacco Quality in Guizhou Province, Guizhou University, Guiyang, 550025, China

Abstract:

Seed priming with gibberellic acid (GA3) could promote seed germination in tobacco; however, the molecular mechanism is poorly understood. In this study, the physiological development and transcriptional regulatory network of tobacco seeds primed with GA3 or water, and non-primed seeds were studied during seed germination. When compared with non-primed or hydro-primed seeds, tobacco seeds primed with 100 mg/L GA3 solution for 24 h showed increased germination speed and uniformity under low temperatures (10°C). GA1+3 levels and ß-1,3-glucanase activity were significantly higher at the germination starting and pre-endosperm-rupture stages respectively. The transcriptome between the GA3-primed seeds and hydro-primed seeds was much similar than to that of the non-primed seeds. The transcripts for which their expression patterns were significantly up- or downregulated in both the GA3-primed and hydro-primed seeds relative to non-primed seeds were the focus of the study, 93.90% of which after priming and 83.89% of which during radicle expansion were regulated in the same manner, respectively. GA3 priming promoted seed germination that was associated with 67 differentially expressed transcripts after priming, 64 of which were associated with radicle expansion. Of these, ABA receptors PYL1 and PYL7, ABA biosynthesis genes ABA1, NCED6, NCED9, and AAO3, ABA negative signal regulators ABI2, AHG3, ABI3, ABI4, and ABI5, auxin response factor ARF10, ARF 16, and ARF17, and light receptors PHYB and negative signal regulators PIF1, and XERICO were induced by GA3 priming. This study, thus, preliminarily revealed the physiological and molecular mechanism of gibberellin-priming promoting seed germination.

Page(s): 1768-1778

DOI: 10.17957/IJAB/15.0693

Published: Journal: International Journal of Agriculture and Biology, Volume: 20, Issue: 8, Year: 2018

Keywords:

Keywords are not available for this article.

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