A validated RP-HPLC method to investigate Finasteride in human skin after in vitro topically applying vesicular nanocarrier. | [Pakistan Journal of Pharmaceutical Sciences • 2014]

Author(s):

1. Feiyue Zheng: Department of Pharmacy, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University, Hangzhou, China

2. Yuefeng Rao: The First Affiliated Hospital, College of Medicine and College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China

3. Yan Lou: The First Affiliated Hospital, College of Medicine and College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China

4. Xiaoyang Lu: The First Affiliated Hospital, College of Medicine and College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, China

Abstract:

The pharmacotherapeutic efficiency of topical drug delivery systems is mainly dominated by the skin distribution of therapeutic agents. In this work, a sensitive, rapid and fully-validated reversed-phase high performance liquid chromatography (RP-HPLC) method was developed to determine finasteride in human cadaver skin after different vesicular formulations were applied. Drug in different depth of skin layers were measured with an EclipseXDB-C18 column. The mobile phase consisted of 75% (v/v) methanol containing 0.2% phosphoric acid buffered to pH 3.0 with triethylamine under isocratic conditions. The system was operated at 40°C and the mobile phase flow rate was set at 1 mL/min. The standard-calibration curve was linear within range of 5 to 200 ng/ml with correlation coefficient 0.9996. The intra-assay precision was less than 3.9% while the inter-assay precision was less than 7.1% with the bias range of - 8.6 to 4.1%. This method was found to be specific, accurate, and sensitive and was successfully used to determine the accumulation of finasteride after in-vitro percutaneous delivery by liposomal or ethosomal drug delivery nanocarriers.

Page(s): 525-529

DOI: DOI not available

Published: Journal: Pakistan Journal of Pharmaceutical Sciences, Volume: 27, Issue: 3, Year: 2014

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